中文摘要：

以微藻油脂提取后的藻渣为底物,研究了厌氧消化污泥预处理方式对产氢的影响,预处理方法为热、碱、酸和氯仿处理。结果表明,污泥预处理方式对藻渣的产氢量和消解率有很大影响。热处理污泥的底物消解率最高,而酸处理时为最低;热处理污泥的氢气产量最高,为27.27 ml/g,酸、碱、氯仿和未处理污泥的氢气产量较低,分别为13.97,23.69,17.73和18.88 ml/g。虽然氯仿处理和酸碱处理完全抑制了甲烷菌活性,但是产氢细菌的活力也受到抑制,其中酸处理的抑制最为严重。因此,热处理是富集产氢细菌最有效的方法。

英文摘要：

Effects of various pretreatment methods of anaerobic digested sludge on hydrogen pro-duction from lipid-extracted microalgal biomass residues were evaluated,the selected pretreatment methods were heat,acid,base,and chloroform,respectively.The results showed that the efficiency of hydrogen production and substrate removal efficiency were dependent on the pretreatment meth-ods used.The highest substrate removal efficiency of 23% was obtained with the heat-treated sludge,while the lowest one of 14% was obtained with the acid pretreated sludge.The highest hydrogen yield of 27.27 ml/g was achieved with heat-treated sludge,whereas the hydrogen yield of the sludge treated by acid,base,chloroform pretreatment and control was 13.97,23.69,17.73 and 18.88 ml/g,respectively.Although the acid,base and chloroform pretreatment completely inhibited the activity of methanogenesis,the activity of hydrogen-producing bacteria was partially suppressed,especially for the acid pretreatment.The heat pretreatment method was proved to be better than the others for enriching hydrogen-producing bacteria from anaerobic digested sludge.