中文摘要：

文章主要针对绿藻蛋白核小球藻（Chlorella pyrenoidosa）进行了有硫和无硫培养，并对其低光强产氢及重复产氢进行了研究。研究发现，蛋白核小球藻在低光强下可持续产氢．且利用重复产氢的方法可以提高蛋白核小球藻的总累积产氢量，延长产氢时间。蛋白核小球藻在有硫和无硫培养条件下的产氢明显不同．在有硫产氢条件下，蛋白核小球藻停止产氢后，可在加入乙酸钠培养后重复产氢：在无硫产氢条件下，蛋白核小球藻可在培养24h后，不需添加乙酸钠而产氢，且经重复培养一次后，第二次产氢阶段的蛋白核小球藻的单次产氢量仍比第一次的产氢量高，说明蛋白核小球藻在有硫和无硫产氢条件下存在两种不同的代谢途径。

英文摘要：

In this study the repeated photobiological Hzproduction of Chlorella pyrenoidosa in sulfur replete and sulfur deprived medium was investigated under low light intensity condition. It was found that total cumulative H2 production of C. pyrenoidosa could be increased by repeated H2 production method. Under sulfur replete condition, C. pyrenoidosa could release H2 again when the alga cells were cultivated in sodium acetate addition medium. Under sulfur deprived condition, C. pyrenoidosa could reproduce H2 after 24 hours cultivation without sodium acetate addition; furthermore, after cultivated once, H2 production of C. pyrenoidosa in second stage was still higher than the H2 production in first stage. These results indicated that there were two different metabolic pathways for C. pyrenoidosa under sulfur deprived and replete condition.