中文摘要：

利用简单重复序列间扩增（ISSR）分子标记对舟山群岛红楠（Machilus thunbergii）8个种群91个个体进行了遗传结构分析，9条随机引物扩增出可分析条带75条。多态位点百分比（PPL）为61．3％。POPGENE分析结果表明：红楠种群平均水平多态位点百分比（PPL）为52．3％，Nei’s基因多样度（HE）为0．186，较台湾岛红楠种群（PPL为71.1％）具有偏低的遗传多样性；而种群间遗传分化程度较高（GST=0．311）。地理距离与遗传距离具有显著相关性（r=0．655，P=96．1％），岛屿地理隔离对红楠种群间遗传分化产生显著影响。UP—GMA聚类分析表明：朱家尖岛与普陀岛红楠种群间遗传相似度较高，桃花岛与大猫岛可能存在较近的亲缘关系。基于舟山群岛红楠种群遗传结构分析，建议在其自然生长地实施就地保护的同时，建立红楠种质资源库，促进基因交流。

英文摘要：

Ninty-one individuals of eight populations of Machilus thunbergii collected From Zhoushan archipelago were examined by inter-simple sequence repeat （ISSR）markers to determine the genetic structure. A total of 75 discernible loci were obtained using 9 primers,of which 61.3% were polymorphic （PPL=61.3 of precintage in genetic div of bolymorphic loci （PPL = 52. %）. As analy 3 % ） indicted ersity compared with Taiwan populations. zed by POPOGENE,average values that M. thunbergii had a reduction The value of differentiation（GST =0. 311）affirmed that M. thunbergii had high genetic differentiation among populations. The effect of geographical isolation had great influences and significant correlation was found between genetic distance and geographic distance （r = 0. 655, P = 96. 1 %）. UPGMA indicated that Putuo Island population showed a close relationship with Zhujiajian Island populations, Damao Island population and Taohua Island populations clustered in a clade. In order to were proposed that natural population shou d be protected in situ from human disturbance to facilitate its natural generation. Constructing germplasm resources and cultivating plants mutually collected from different populations are necessary to enhance the gene flow.