中文摘要：

通过感受态细胞的生长状态、转化温度及不同的电击杯内径、电压等影响电转化的重要条件，探讨索了大肠杆菌JM109菌株电转化的最优条件．在感受态细胞OD600值为0．5-0．7，转化环境温度为4℃，电容25μF、并联电阻200Ω、电压2．5kV和0．2cm电击杯电转化效率最高，可达到9．12×10^8．

英文摘要：

To find out the optimized conditions for electroporation of Escherichia coli JM109, the optimal electroporation protocol would be found out through changing some important effecting factors, such as the growth phrase of the germ, temperature, voltage, etc. It showed that the optimal conditions were cells harvested in OD600 0. 5--0. 7, capacitance 25 μF, electric resistance 200 Ω, voltage 2. 5 kV, cuvette with an interelectrode distance of 0. 2 cm. Under this condition, the transformation efficiency is 9.12 ×10^8.