中文摘要：

目的 运用iTRAQ定量蛋白质组学技术筛选慢性浅表性胃炎（chronic superficial gastritis,CSG）不同中医证候患者唾液中的潜在特异性生物标志物,探索一种客观化、规范化的中医辨证方法 .方法采用iTRAQ定量蛋白质组学技术及后期生物信息学方法,筛选CSG脾虚证28例与CSG湿热证28例对比以及与正常对照组28例对比的唾液差异蛋白质分析.结果 CSG脾虚证对比CSG湿热证,Ratio≥1.5的共有128个蛋白,Ratio≤0.667的共有39个蛋白;CSG脾虚证对比正常对照组Ratio≥1.5的共有113个蛋白,Ratio≤0.667的共有72个蛋白.筛选出多个重要蛋白,如Keratin家族、ANX家族和apolipoprotein家族中的多种亚型以及γ-谷氨酸转移酶等,可能与CSG不同中医证候的发生、发展有密切的关系.结论 iTRAQ唾液蛋白质组技术筛选出了多个具有潜在价值的CSG中医证候无创诊断的重要蛋白,值得进一步深入研究.

英文摘要：

Objective To screen the potential specific biomarker in saliva of chronic superficial gastritis （CSG） with different TCM syndromes by iTRAQ quantitative proteomics, and to explore the objective and standard TCM syn-dromes differentiation methods. Methods Saliva samples were taken from 28 cases of CSG patients with spleen deficiency syn-drome （group A）, 28 cases of CSG patients with damp-heat syndrome （group B） and 28 cases of normal control volunteers （group C）, in which differential expressed proteins were analyzed by iTRAQ quantitative proteomics and bioinformatics methods. Results 1.In the comparison between group A and group B, it screened out 128 up-regulated proteins （ratio≥1.5） and 39 down-regulated proteins （ratio≤0.667）. 2. In the comparison between group B and group C, it screened out 113 up-regu-lated proteins （ratio≥1.5） and 72 down-regulated proteins （ratio≤0.667）. 3. This study screened out a large number of differential expression proteins in saliva, including various subtypes in Keratin family, ANX family, apolipoprotein family, and γ-glutamyltrans-ferase, which may be related to the occurrence and development of different syndromes of CSG. Conclusion The multiple poten-tial molecular biomarkers in saliva to diagnose CSG with different TCM syndromes were screened by iTRAQ quantitative pro-teomic technology, which is worthy of further study.