中文摘要：

背景：近来骨髓基质细胞多向分化潜能的发现，特别是骨髓基质细胞向神经干细胞方向分化的成功，为干细胞移植治疗神经系统损伤提供了可能。目的：观察自体骨髓基质源干细胞对脑损伤的治疗作用。设计：以BrdU标记的自体骨髓基质源干细胞在模型动物损伤脑区和其他脑区的分布为观察对象的前瞻性研究。单位：北京军区总医院。材料：实验于1999-10／2002—02在北京军区总医院完成。取1．5～3．0岁成年家犬22只，雌雄不限，体质量（13±2）kg。随机分为假手术组6只，常规治疗组8只，骨髓基质源神经干细胞组8只。方法：对犬骨髓基质细胞进行采集、分离、体外扩增、标记和移植用细胞培养。假手术组仅做皮肤切口及颅骨锥孔，不致中脑损伤，于麻醉清醒后行正常进食。常规治疗组行中脑被盖区毁损后行三磷酸腺苷20mg，辅酶A50U，加入生理盐水中静脉滴注，2次／d，共3d。骨髓基质源神经干细胞组行中脑被盖区毁损，然后给予骨髓基质细胞源神经干细胞1mL和脑脊液1mL的混合液，注入脑室，1次／d，共3d。术后第1，7，14，28天4个时间点观察骨髓基质源干细胞在损伤脑区及其他脑区的分布情况。主要观察指标：观察骨髓基质源干细胞在损伤脑区及其它脑区的分布情况。结果：22只动物均进入结果分析。①在术后第28天：骨髓基质源神经干细胞组BrdU阳性细胞明显高于假手术组和常规治疗组[（15．4±2．4，0．5±0．5，0．5±0．5）个1。②骨髓基质源神经干细胞组术后第14天脑片BrdU阳性细胞数：中脑的伤侧明显高于对侧[（15．5±3．3，5．0±1．5）个]；额叶的伤侧高于对侧[（1．6±0．6，1．5±0．7）个]。结论：由于骨髓基质源神经干细胞移植组BrdU阳性细胞数在脑损伤区的分布明显多于对侧非伤区和两个对照组的同部位。提示骨髓基质源干细胞可?

英文摘要：

BACKGROUND： In recent years, the discovery that bone marrow stromal cells （BMSCs） have the potential of muhi-differentiation, especially the successful differentiation of BMSCs to neural stem ceils （NSCs）, has provided possibility for cell transplantation in treating injury of nervous system. OBJECTIVE： To observe the therapeutic effect of the anrotransplantation of BMSCs-derived stem cells on brain injury. DESIGN： A prospective study by taking the distribution of BrdU labeled autologous BMSCs-derived stem cells in the damaged and other brain areas of animal models as the observational object. MATERIALS： The experiments were carried out in the General Hospital of Beijing Military Area Command of Chinese PLA from October 1999 to February 2002. Twenty-two adult dogs of 1.5-3.0 years old, either males or females, weighing （13±2） kg, were randomly divided into sham-operated group （n=6）, routine treatment group （n=8） and BMSCs-derived stem cells group （n=8）. METHODS： Dog BMSCs were collected proliferated, separated, pre-differentiated in vitro, marked and cultured. In the sham-operated group, the dogs were only treated with skin incision and skull drilling, but not induced to midbrain injury, and they could eat normally after became conscious from anesthesia. After lesion of midbrain tagmental area, in the routine treatment group, adenosine triphosphate （20 mg） and coenzyme A （50 u） were added to salineand then dripped via vein, twice a day for 3 days; In the BMSCs-derived NSCs group, the mixture of 1 mL BMSCs-derived NSCs and 1 mL cerebrospinal fluid was infused into the brain ventricle, once a day for 3 days continuously. The distribution of BMSCs-derived stem cells in the damaged and other brain areas was observed on the 1^st, 7^th, 14^th and 28^th days postoperatively. MAIN OUTCOME MEASURES： The distribution of BMSCs-derived stern cells in the damaged and other brain areas was observed. RESULTS： All the 22 dogs were involved in the analysis of results. ① On the 28^t