以壳聚糖改性凹凸棒土作为普通小球藻Chlorella vulgaris的絮凝剂,CaCl2为助凝剂,研究了絮凝采收小球藻的工艺条件。结果表明,最佳絮凝条件为:絮凝剂0.4g/L、壳聚糖/凹凸棒土为1:12、助凝剂CaCl2为0.2g/L、溶液的pH为9.0,此条件下小球藻絮凝率达到95%以上。絮凝剂的扫描电镜图和BET比表面积(BET)数据显示,改性后的凹凸棒土以一定的空间结构状吸附连接壳聚糖,有效增加了絮凝剂的比表面积,有利于小球藻的吸附。研究采用的原料价格低廉、安全环保,絮凝剂的制备简单且絮凝效率高,可广泛用于工业化生产中微藻的采收。
Chitosan modified attapulgite is used as the flocculant for precipitating Chlorella vulgaris with adding CaCl2 as the coagulant. For Chlorella vulgaris, the optimal flocculation conditions showed as following: flocculant is 0.4g/L, the ratio of chitosan and attapulgite in flocculant is 1:12, CaCl2 coagulant dosage is 0.2g/L, and pH of the solution is 9.0. Under the best conditions, the flocculation rate of Chlorella vulgaris can reach up to 95%. The scanning electron micrographs and the BET specific surface area data(BET) of flocculant show that the modified attapulgite adsorbs and connects with chitosan to a certain degree of spatial structure, which effectively increases the surface area of flocculant and is conducive to the adsorption of Chlorella. The raw materials used in this article could have the advantages of low cost, safety and low impact on the environment. And the high efficiency of flocculation can obtain with sample method of preparing the flocculant, which can be widely used for the harvest of microalgae in industrial production.