中文摘要：

目的 通过检测姊妹染色体凝集和分离相关蛋白染色体结构维持蛋白（SMC）1、SMC3、分离酶（Separase）和保全素（Securin）在煤焦沥青致BEAS-2B癌变细胞中的变化,探讨其在煤焦沥青接触者肺癌发生中的作用.方法 用中温煤焦沥青烟提取物作为诱导剂,建立永生化人支气管上皮细胞BEAS-2B的恶性转化模型,用实时定量反转录-聚合酶链反应（RT-PCR）检测相关基因的mRNA表达水平;细胞爬片免疫组化半定量法检测其蛋白表达改变.以二甲亚砜（DMSO）溶剂组和未染毒的正常传代的BEAS-2B细胞为对照组.结果 与正常对照组和DMSO溶剂对照组比较,煤焦沥青烟提取物诱导转化细胞中,SMC1基因和蛋白表达水平的差异无统计学意义（P＞0.05）;煤焦沥青烟诱导组SMC3和Separase的基因和蛋白表达水平明显升高（基因：SMC3：2.54±0.20、Separase：2.42±0.22,蛋白：SMC3：0.27±0.02、Separase：0.25±0.02）,Securin的基因和蛋白表达水平明显降低（基因：0.30±0.04,蛋白：0.17±0.01）,与其他两组（DMSO溶剂对照组：基因：SMC3：1.13±0.12、Separase：1.00±0.04、Securin：0.90±0.11,蛋白：SMC3：0.10±0.03、Separase：0.18±0.02、Securin：0.22±0.02;正常对照组：基因：SMC3：1.00±0.11、Separase：1.00±0.08、Securin：1.00±0.11,蛋白：SMC3：0.09±0.01、Separase：0.18±0.01、Securin：0.23±0.02）相比,差异均有统计学意义（P＜0.05）.结论 在煤焦沥青致支气管上皮细胞恶性转化过程中,姊妹染色单体凝集和分离相关蛋白SMC3和Separase升高,Securin表达水平降低,参与了细胞恶性转化过程.

英文摘要：

Objective To study the role of structural maintenance of chromosome （SMC）1, SMC3,Separase and Securin in tumorgenesis that contact with coal tar pitch. Methods The BEAS-2B cells was induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, and the protein expression variation were determined by cell culture overslip of immunohistochemical methods. Results In malignant transformation cells, the mRNA and the protein expression level of SMC1 gene was not statistically significantly different compared with the BEAS-2B group and DMSO group （P〉0.05）; SMC3 and Separase was increased and Securin was decreased （P〈0.05） ,while the difference between other two control groups was not significant （P〉0.05）. Conclusions The up expression level of SMC3 and Separase and the down expression level of Securin are involved in the process that evolves into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.