中文摘要：

目的：检测p53基因第4外显子的第72密码子Arg/Pro多态性及吸烟对河南汉族人肺癌发病的影响。方法：采用PCR-限制性片段长度多态性技术检测124例肺癌患者和128名对照（病例和对照均为河南汉族人）p53基因Arg/Pro多态性,同时调查两组人群的吸烟状况,比较基因多态性和吸烟在两组中的分布情况。结果：对照组和肺癌组人群Pro/Pro基因型频率分别为15.6%和28.2%,与携带Arg/Arg和Arg/Pro基因型者比较,携带Pro/Pro基因型个体患肺癌危险增加且其调整OR（95%CI）为2.11（1.12～3.97）。以不吸烟和非Pro/Pro基因型者为参照,Pro/Pro基因型和吸烟单独作用的调整OR（95%CI）分别为0.93（0.36～2.38）和2.27（1.13～4.54）,二因素共同存在时的调整OR（95%CI）为10.49（3.42～32.23）,Pro/Pro基因型与吸烟存在交互作用（χ2=5.209,P=0.023）。结论：吸烟、p53Pro/Pro基因型及其二者交互作用可能是河南汉族人群肺癌发病的危险因素。

英文摘要：

Aim：To investigate the effect of Arg/Pro polymorphism of p53 within codon 72 in exon 4,smoking,and their combined effect on the occurrence of lung cancer in Henan Han population.Methods：Arg/Pro polymorphism of p53 and smoking states were compared among 124 patients with lung cancer and 128 normal controls.Arg/Pro polymorphism was detected by PCR-RFLP.Results：Frequencies of Pro/Pro genotype were 15.6% in controls and 28.2% in cases,respectively;compared with Arg/Arg and Arg/Pro genotype,Pro/Pro genotype increased the risk of lung cancer and the adjusted OR（95%CI） was 2.11（1.12～3.97）.Compared with non-Pro/Pro genotype and non-smoking,the adjusted OR（95%CI） of Pro/Pro genotype and smoking was 0.93（0.36～2.38） and 2.27（1.13～4.54）,respectively.For the individuals with smoking and Pro/Pro genotype,the adjusted OR（95%CI） was 10.49（3.42～32.23）.Interaction between smoking and Pro/Pro genotype was observed （χ2=5.209,P=0.023）.Conclusion：Smoking,p53 Pro/Pro genotype and their interaction may contribute to the occurrence of lung cancer in Henan Han population.