用He-Ne激光(5.23mW·mm^-1)处理经5%、10%、15%PEG6000胁迫的小麦幼苗,分析了干旱胁迫条件下激光处理对小麦幼苗保护酶活性及脂质过氧化作用的影响。适度干旱胁迫的小麦幼苗经He-Ne激光辐照后,丙二醛(MDA)含量和超氧自由基(O2^-)产生速率显著降低p〈0.05),而过氧化物酶(POD)活性和抗坏血酸(AsA)、谷胱甘肽(GSH)含量却显著增加(P〈0.05)。总体上看,5%和10%PEG6000胁迫的小麦幼苗经激光辐照3min后抗旱性增强。
Aims We studied protective enzyme activities and lipid peroxidation in wheat seedlings treated with different concentration of PEG6000 to determine the effects of He-Ne laser on wheat (Triticum aestivum) exposed to drought stress. Methods We treated 12-day-old, drought-stressed seedlings with 5%, 10% and 15% PEG6000 for 3, 6, 9, 12 and 15 days. After 3, 6, 9, 12 and 15 days of drought stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0, 1 and 3 min. Leaves were sampled for various analyses. Important findings He-Ne laser irradiation significantly decreased malondialdehyde (MDA) content and superoxide radical (02) production rate and notably increased peroxidase (POD) enzyme activity and ascorbate (AsA) and glutathione (GSH) concentration in wheat seedlings exposed to moderate drought stress. We concluded that 3 min laser irradiation enhanced anti-drought characteristics of wheat seedlings treated with 5% or 10% PEG6000.