中文摘要：

治疗学的克隆，胚胎的干细胞(转换字符) 由此经由体的房间 nuclear 转移(SCNT ) 从病人特定的克隆的胚囊被导出，为用再生药治疗许多人的疾病的抓住伟人诺言。Teratoma 形成和细菌线传播被用来证实老鼠干细胞的 pluripotency，但是人的胚胎的干细胞(hESCs ) 没被证明是充分由于为细菌测试的道德的不可能的 pluripotent 衬里传播，它将是为完整的 pluripotency 的最充分的证据。因此，从在 teratoma 以内的三体的细菌层的区分的房间的形成在 hESC 线作为 pluripotency 的最好的指示物被拿。这些线缺乏的可能性完整多 -- 或 pluripotency 还没被评估了。在这研究，我们建立了 16 根老鼠转换字符线，包括 3 遗传上有缺点的原子转移 -- 转换字符(ntESC ) 线源于不肥沃的雌雄同体 F1 老鼠和从正常 F1 老鼠的 SCNT 胚囊导出的 13 根 ntESC 线的 SCNT 胚囊。我们发现有缺点的 ntESCs 在 pluripotency 的 vitro 标记表示了所有并且能形成从所有三细菌层包括了衍生物的 teratomas，但是不能经由细菌线被播送，在有正常的对比 ntESCs。我们的结果显示有 hESCs 的 teratoma 形成试金可能是一个不够的标准估计完整的 pluripotency，尽管他们确实多在某种程度上定义力量。更严密的标准被要求为治疗学的克隆估计 hESCs 的安全。

英文摘要：

Therapeutic cloning, whereby embryonic stem cells （ESCs） are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer （SCNT）, holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells （hESCs） have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmis- sion, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi- or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer- ESC （ntESC） lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results in- dicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning.