中文摘要：

【目的】建立一种新型的、能在基层场地进行实时便捷诊断的猪瘟病毒检测方法。【方法】环介导等温扩增技术（Loop-mediated isothermal amplification,LAMP）-横向流动试纸条技术（Lateral flow dipstick,LFD）相结合,针对猪瘟病毒（Classical swine fever virus,CSFV）NS5B基因设计一套特异性引物及生物素标记探针,结合异硫氰酸荧光素标记的扩增产物,建立RT-LAMP-LFD检测方法。【结果】所建立的方法能够特异地检测出CSFV的存在,检测PRRSV、JEV、PCV-2等其他病毒均为阴性;所建立的CSFV-RT-LAMP方法对RNA的最低检测限为50 pg;反应快速,整个扩增反应可在1 h内完成;操作简单,不需要PCR仪等复杂的仪器,结果可经肉眼观察。【结论】应用RTLAMP-LFD检测CSFV特异性强、灵敏度高,并且操作安全、简便、快捷,可以满足基层检疫的需要。

英文摘要：

【Objective】The experiment was conducted to establish a rapid real time detection method of classical swine fever virus at local fields. 【Method】A rapid detection method（ RT-LAMP-LFD） of classical swine fever virus（ CSFV） was developed by loop-mediated isothermal amplification（ LAMP） combined with lateral flow dipstick（ LFD） method. The LAMP method for amplification of the CSFV NS5 B gene using fluorescein isothiocyanate-labeled primer was combined with a chromatographic LFD for rapid and simple visual detection of CSFV. 【Result】This method could specifically detect CSFV,but not other virus such as PRRSV,JEV and PCV-2. The minimum detection limit of the CSFV-RT-LAMP-LFD method was 50 pg. The detection could be finished within 1 h without using complex instruments such as PCR machine and the results were visible by eye. 【Conclusion】This RT-LAMP-LFD method is a highly specific and sensitive,safe,rapid and simple way for field detection of CSFV infection.