中文摘要：

【摘要】目的研究神经上皮细胞转化基因1（NET1）在电离辐射损伤反应中的生物学功能。方法运用RNA干扰技术，在细胞中特异性沉默Netl基因，然后通过集落存活实验和免疫印迹法研究抑制Net1表达对细胞的电离辐射损伤反应的影响。结果Netl敲低表达的细胞对电离辐射的敏感性增强，表现为更多细胞发生了凋亡，并且在γ射线照射后，毛细血管扩张性共济失调症突变蛋白（ATM）和细胞周期检查点激酶2（Chk2）的磷酸化水平也比对照细胞增强。结论Net1对于受到电离辐射的细胞有一定的保护作用，很可能在电离辐射损伤修复中具有重要作用。

英文摘要：

Objective To study the biological roles of the neuroepithelioma transforming gene 1 （Netl） in the cellular responses to ionizing radiation （IR）-induced damage. Methods Specific shRNA was used to deplete Netl in cells. The effects of Netl depletion on the cellular responses to ionizing radi- ation were investigated through the colonogenic survival assay and immunoblotting analysis of DNA dam- age response proteins＇ phosphorylation. Results Netl-depleted cells were more sensitive to IR, with a significantly increased induction of apoptosis. In response to IR, the phosphorylation levels of ataxia- telangieetasia mutated and checkpoint kinase 2 were much higher in the Netl-depleted cells than that in the control cells. Conclusion Netl protects IR-treated cells from apoptosis and possibly plays an impor- tant role in /R-induced damage response and repair.