中文摘要：

旨在研究小型猪复合麻醉剂（XFM）对大鼠不同脑区LKB1基因mRNA转录和p-LKB1蛋白表达的影响。将30只SD大鼠随机分成XFM组（M组）和生理盐水对照组（C组）,M组又按照时间点的不同分为4个亚组。各组大鼠到达试验设计时间点后分别采取脑组织并分离各脑区,采用PCR和Western blot技术,分别检测各试验组中大鼠不同脑区LKB1基因mRNA的相对表达量和p-LKB1蛋白表达量。结果显示：在试验的麻醉早期阶段（即M1和M2）,各脑区LKB1基因mRNA转录与对照组相比均未出现显著变化（P〉0.05）;大脑皮层、海马及小脑p-LKB1蛋白表达与对照组比较并无显著变化（P〉0.05）,而丘脑与脑干p-LKB1蛋白表达与对照组比较则明显升高,且丘脑差异显著（P〈0.05）,脑干差异极显著（P〈0.01）。而在麻醉后期阶段（即M3和M4）各脑区LKB1基因mRNA转录表达明显上升,尤以M4突出,差异极显著（P〈0.01）,其中丘脑和脑干变化最为明显;大脑皮层、海马及小脑p-LKB1蛋白表达与对照组比较均无明显变化（P〉0.05）,但丘脑和脑干p-LKB1蛋白表达呈现显著升高（P〈0.05）。研究表明,XFM麻醉作用可能影响大鼠中枢神经系统中LKB1基因mRNA的转录和p-LKB1蛋白的表达。

英文摘要：

The aim of this experiment was to investigate the effects of miniature pig compound anesthetic XFM on the expression of LKB1 mRNA and p-LKB1 in different brain regions of rats. 30 SD rats were randomly divided into XFM（ M） and saline control（ C）,and M groups. Group M was divided into 4 sub-groups according to the different time points. At the experimental design time point,the brain tissue was taken to separate the brain regions. The relative expression of LKB1 mRNA and p-LKB1 protein in different brain regions of rats in eachexperimental group was detected by RT-PCR and Western blot. The results showed that the transcription of LKB1 mRNA in different brain regions did not change significantly（ P〉0. 05） compared with the control group. The expression of p-LKB1 protein in cerebral cortex,hippocampus and cerebellum had no significant changes（ P〉0. 05）,while the expression of p-LKB1 protein in thalamus（ P〈0. 05） and brain stem（ P〈0. 01） were significantly higher than that in control group at early experimental stages（ M1 and M2）. The transcription of LKB1 mRNA was significantly increased（ P〈0. 01） in the brain regions,especially in thalamus and brain stem in M4. However,at this time,the expression of p-LKB1 protein showed no significant difference（ P〉0. 05） in the cerebral cortex,hippocampus and cerebellum. But the expression of p-LKB1 protein in the thalamus（ P〈0. 05） and brain stem（ P〈0. 01） showed a significant elevation（ P〈0. 05）. The results indicated that the anesthetic effect of XFM might have an effect on the expression of LKB1 mRNA and p-LKB1 protein in different brain regions in rats.