中文摘要：

目的探讨干扰OVA66基因表达对移植瘤细胞生物学功能的影响。方法采用脂质体法，以重组的pSUPER-shRNA-OVA66和空载体转染HeLa细胞获得稳定表达细胞株，经RT-PCR和Western blot鉴定OVA66基因的表达。将两组细胞接种于BALB／cnu／nu裸鼠的前肢腋下，连续观察干扰OVA66表达对肿瘤的生长影响。并于接种4周后，应用RT-PCR，免疫组化、免疫荧光等方法检测移植瘤细胞中OVA66的蛋白表达。组织病理分析肿瘤细胞生长和转移的特征。结果经检测pSUPER-shRNA-OVA66能有效抑制目的基因的表达。比较两组荷瘤小鼠中肿瘤的生长曲线发现，OVA66实验组的肿瘤生长速度明显低于对照组，并且肿瘤细胞体内转移和浸润能力显著降低。结论干扰OVA66基因和蛋白表达，可以抑制肿瘤细胞的生长，浸润和侵袭能力。

英文摘要：

Objective To investigate the biological effects of OVA66 on tumor migration and invasion in experimental tumor models. Method OVA66 expression was knocked down by small interference RNA （siRNA） in HeLa cells. 2 × 10^6 OVA66-knockdown HeLa cells were injected into both flank of four week-old BALB/c^nu/nu mice. Xenograft tumors were harvested after 35 days and measured by caliper. The tumor volume was quantified using the following formula： volume = length x width^2/2. Samples of the lungs were taken and stained with hematoxylin and eosin for quantification of total metastatic lesions. OVA66 expression was detected in tumor sections by histological analysis. Results OVA66 knockdown significantly slowed the formation of tumor in Balb/C nude mice, resulting in remarkably smaller tumor volume at 5 weeks after tumor cell inoculation. Furthermore, low expression of OVA66 in HeLa cells significantly diminished lung metastasis in the xenograft mice. Compared with the mock control, OVA66 expression in xenografts was decreased up to 70% in OVA66-knockdown group. Conclusion Our results indicate that interference of the expression of tumor-associated antigen OVA66 gene and protein may inhibit the growth, migration and invasion activity of tumor cells in vivo.