中文摘要：

目的观察化风丹对小胶质细胞介导的神经炎症反应的抑制作用。方法采用原代大鼠小胶质细胞培养,通过脂多糖（LPS）诱导小胶质细胞激活引起神经炎症反应。实验随机分为空白对照组、化风丹组（0.3 mg/mL）、模型组（10 ng/mL LPS）、LPS＋化风丹组（0.03、0.1和0.3 mg/mL）。实时反转录聚合酶链反应（Real-timeRT-PCR）检测细胞中炎症因子mRNA的表达,酶联免疫吸附试验（ELISA）和Griess试剂检测细胞培养上清液中炎症因子蛋白含量的变化。结果化风丹能够抑制LPS诱导的小胶质细胞内肿瘤坏死因子α（TNFα）、白介素-1β（IL-1β）和诱导型一氧化氮合酶（iNOS）mRNA的过度表达以及降低细胞培养上清液中TNFα、IL-1β和一氧化氮（NO）的含量。结论化风丹能够抑制小胶质细胞介导的神经炎症反应。

英文摘要：

Objective To investigate the inhibitory effects of Hua Feng Dan（HFD） on microglia-mediated neuroinflammation.Methods Primary rat microglia cultures were used and treated with lipopolysaccharide（LPS） to induce neuroinflammation.The cultures were randomly divided into control,HFD（0.3 mg/mL）,LPS（10 ng/mL） and LPS＋HFD（0.03,0.1 and 0.3 mg/mL） groups.Real-time RT PCR was performed to measure the mRNA expressions of microglial inflammatory factors.ELISA and Griess reagent were applied to detect the production of inflammatory factors in the supernatant of cultures.Results HFD inhibited LPS-induced mRNA overexpressions of tumor necrosis factor-α（TNFα）,Interleukin-1β（IL-1β） and inducible nitric oxide synthase（iNOS） in microglia and the production of TNFα,IL-1β and nitric oxide（NO） in the culture supernatant.Conclusion HFD could inhibit miroglia-mediated neuro-inflammation.