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Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening

ISSN号：1000-4718
期刊名称：《中国病理生理杂志》
时间：0
分类：R735.1[医药卫生—肿瘤;医药卫生—临床医学]
作者机构：[1]Beijing Union Med Coll, Inst Canc, Dept Cell Biol, Natl Lab Mol Oncol, Beijing 100021, Peoples R China, [2]Chinese Acad Med Sci, Beijing 100021, Peoples R China
相关基金：This work was co-supported by the State Key Basic Research Program (Grant Nos. G1998051021 and G1998051205); and the National Natural Science Foundation of China (Grant Nos. 39993420 and 39990570).

作者： Jin Zhou, Zhihua Liu, Xiuqin Wang, Fang Ding, Chuannong Zhou, Min Wu

关键词： ESOPHAGEAL, cancer, suppression, subtractive, HYBRIDIZATION, (SSH), differential, expression, lipocortin, cystatin., esophageal cancer, suppression subtractive hybridization (SSH), differential expression, lipocortin, cystatin

中文摘要：

To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression sub-tractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such as liporcotin I, cystatin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with PC

英文摘要：

To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression subtractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such asliporcotin I,cystalin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with PCR-based doublestrand cDNA synthesis and high throughput reverse Northern screening is an efficient way to isolate differentially expressed genes from microgram of total RNA.

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