中文摘要：

为了应用RNAi技术调控杏自交不亲和性状,根据自交不亲和S66-RNase基因序列（Gen Bank登录中）设计特异引物,通过RT-PCR扩增S66-RNase基因（Gen Bank登录中）高变区及下游（63 bp）作为靶基因,构建RNAi表达载体的ihp RNA。以植物表达载体p CAMBIA为骨架载体,利用农杆菌介导的方法转化本氏烟,利用子房转化法转化小白杏。结果显示：获得了长度为439 bp的ihp RNA。表达载体酶切检验表明S66-RNase基因的RNAi表达载体构建和转入农杆菌LBA4404成功。通过抗性筛选和GUS染色检测,获得了16株阳性植株。结论：获得转基因烟草植株证明了本研究构建的RNAi植物表达载体有效,为诱导杏S-RNase基因转录后基因沉默、获得自交亲和的杏提供参考。

英文摘要：

We aimed to regulate self-incompatibility in apricot by RNAi technology in this study. The hypervariable region and downstream（63 bp） of S66-RNase gene based on the S66-RNase gene sequence of ＇Xiaobaixing＇ apricot was selected as target gene to construct ihp RNA of RNAi expression vector, which was amplified with the specific primers by PCR method. The expression vector p CAMBIA was used as backbone vector. The result showed that RNAi vector was transformed into Nicotiana benthamiana by Agrobacterium-mediated transformation. The length of ihp RNA was 439 bp, and RNAi expression vector of S66-RNase gene was constructed and transferred into LBA4404 successfully. 16 transgenic plants were obtained by means of resistance screening and GUS staining detection. This study provided a basis for inducing posttranscriptional gene silencing in the transformed plants and obtaining self-compatibility apricot.