中文摘要：

目的研究白头翁皂苷D（毛茛科植物白头翁Pulsatillachinensis（Bge．）Regel的干燥根）体内抗小鼠H22肝癌细和体外的抗人肝癌SMMC-7721作用。方法在体外实验中，采用MTT法考察白头翁皂苷D对SMMC-7721细胞增殖的抑制作用；在体内实验中，采用小鼠H22实体瘤进行体内抑瘤实验，测定肿瘤生长抑制率，利用光镜（HE染色）、电镜等方法进行形态学观察。结果白头翁皂苷D处理人肝癌SMMC-7721细胞24、48、72h后，给药组均出现了不同程度的细胞增殖抑制。白头翁皂苷D（4．5、6．0mg／kg）对小鼠H22肝癌细胞实体瘤的生长有明显的抑制作用，抑瘤率分别为38．67％和41．59％（P〈0．01，与模型组比较）。组织学观察显示，肿瘤细胞大量变性坏死，电镜下可见凋亡细胞和凋亡小体。结论白头翁皂苷D具有一定的体内、外抗肿瘤作用。

英文摘要：

AIM To study the effect of Pulsatilla saponin D from root of Pulsatilla chinensis （Bge.） Regel on human hepatocarcinoma cell line SMMC-7721 in vitro and mouse H=hepatoma carcinoma cell in vivo. METHODS In vitro experiments, MTF assay was used to evaluate proliferational inhibition of P. saponin D on SMMC-7721 cells. In vivo experiments, ICR mice were used to establish H22hepatoma carcinoma cell tumor model. The sup- pression ratio of I-I22 hepatoma carcinoma cell growth was determined. The morphologic and apoptotic body were evaluated by light microscope （HE staining） and electron microscope. RESULTS Medication groups showed dif- ferent levels of SMMC-7721 cells proliferation inhibition after 24 h, 48 h, 72 h with P. saponin D. Marked inhibi- tory effect of Pulsatilla saponin D on the transplanted hepatocellular carcinoma H= was observed in the tumor-bear- ing mice. The inhibitory rates were 38.67% and 41.59% respectively at the dose of 4.5 and 6.0 mg/kg P. sapo- nin D, respectively. （P 〈 0.01 vs. model group）. Histopathologicat examination revealed widespread necrosis with massive accumulation of infiltrating lymphocytes and plasmacytes in the tumors. Numerous apoptotic cells and apop- totic bodies were observed in the tumors under the electron microscope. CONCLUSION Pulsatilla saponin D has anti-tumor effects in vivo and in vitro.