中文摘要：

建立同时测定大鼠血浆中白藜芦醇苷及其代谢产物白藜芦醇的液相色谱-串联质谱方法。以Lichrospher C18色谱柱为分析柱，乙腈-水为流动相，采用电喷雾离子源（ESI），以多反应监测（MRM）模式检测，内标法定量，用于定量分析的离子反应分别为m／z389／227（白藜芦醇苷）和m／z227／143（白藜芦醇）。血浆中的白藜芦醇苷及白藜芦醇用乙酸乙酯提取，N2吹干乙酸乙酯，残留物用甲醇溶解，注入LC／MS／MS系统进行检测。在选定的样品预处理、色谱及质谱条件下，白藜芦醇苷、白藜芦醇及内标物能够达到基线分离而且离子化效果好。用LC／MS／MS法检测大鼠血浆中的白藜芦醇苷及其代谢产物白藜芦醇，线性范围0．4～200μg／L，日内、日间精密度（RSD）均小于15％；检测血浆低、中、高3个浓度（1、20、100μg／L）白藜芦醇苷的回收率分别为106．2％、97．8％和91．6％；检测血浆低、中、高3个浓度（1、20、100μg／L）白藜芦醇的回收率分别为113．2％、103．6％和93．4％。本方法具有灵敏、准确、快速的特点，可用于白藜芦醇苷的药代动力学研究。

英文摘要：

A LC/MS/MS method was used to determine polydatin and its metabolite in Sprague-Dawley （SD） rat plasma. The polydatin and resveratol in plasma were extracted twice by vortexing with 2.0 mL acetoacetate. The organic phase was removed into the tubes and then evaporated in the Zymark TurboVap LV Evaporator. The residue was dissolved in 50 μL methanol. 10μL solution was drawn and detected by LC/MS/MS. Lichrospher C18 was used （150 mm ×2.1 mm, 5μm） with the mobile phase gradient elution of acetonitrilewaters. Electrospray ionization-mass spectrometry （ESI^-） source was used as detector and was operated in negative ion mode selected multiple reactions monitoring （ MRM ） mode with the transitions of m/z 389/227 （polydatin） and m/z 227/143 （resveratrol） used as quantifying, respectively. Under the optimized LC/MS/ MS conditions, polydatin, resveratol and internal standard can be well separated, and their ionization efficiency was satisfactory. The standard curve of polydatin and resveratrol were linear in the concentration range of 0. 4-200μg/L. The recoverier of analytical method for polydatin were 106.2% ,97.8% and 91.6%, for resveratrol were 113.2% ,103.6% and 93.4% when the plasma drug concentration was 1, 20 and 100μg/L respectively. Intra-day and inter-day precises expressed by relative standard derivation （RSD） were less than 15 %. The assay method was sensitive, accurate, fast and suitable to determine the polydatin and its metabolite in SD rat plasma.