中文摘要：

目的观察积雪草苷（aisaticoside，AS）对小鼠腹腔注射脂多糖（lipopolysaccharide，LPS）诱导痛觉增敏型疼痛的影响，并探讨AS对髓过氧化物酶（myeloperoxidase，MPO）、一氧化氮（nitrogen monoxidum，NO）和α-肿瘤坏死因子（α-tumour necrosis factor，TNF-α）的影响。方法随机将小鼠分为空白组、模型组、盐酸哌替啶组、AS5，15，45mg·kg^-1 6组，通过腹腔注射LPS100μg·kg^-1建立痛阈增敏型疼痛模型，采用醋酸扭体法、热板法观察AS的镇痛效应；通过相关试剂盒检测小鼠脑组织MPO和NO，ELISA法检测血浆TNF-α含量的变化。结果AS5，15，45mg·kg^-1各组明显减少醋酸扭体次数，提高小鼠痛闽。降低脑组织MPO活性、NO含量和血浆TNF-α含量。结论AS具有明显镇痛效应，其镇痛效应可能与降低MPO、NO和TNF-α有关。

英文摘要：

OBJECTIVE To observe the analgesia effect of aisaticoside on hyperalgesia induced by intraperitoneal LPS in mice, and investigate the change of MPO,NO and TNF-α. METHODS Mice were divided randomly into normal group,model group, spas-medal group and AS 5,15, 45 mg·kg^-1 group, hyperalgesia was induced by intraperitoneal administration of LPS 100 μg·kg^-1 in mice. The analgesia effect of AS was assessed by the hot-plate stimulation and the writhing test induced by acetic acid,the activity of MPO, NO in brain tissue was tested by kits ; the content of TNF-α in plasma was tested by ELISA. RESULTS AS doses of 5,15,45 mg·kg^-1 significantly reduced the number of writhing, increased pain threshold, decreased the activity of MPO, NO and TNF-α. CONCLUSION AS have the effect of analgesia and its effect maybe related with inhibiting the MPO, NO and TNF-α.