中文摘要：

目的探索积雪草苷的解热作用。方法雄性SD大鼠56只，随机平均分为正常组、模型组、溶媒对照组、对乙酰氨基酚组和积雪草苷3个剂量组。除正常组外，每只大鼠ip细菌脂多糖（LPS）100μg·kg^-1制备大鼠发热模型，连续6h监测大鼠体温变化，6h后取血、肝脏和脑组织标本。积雪草苷3个剂量组造模前连续3dig积雪草苷（5，15和45mg·kg^-1），每日1次；正常组和模型组给予等量生理盐水；溶媒对照组给予等量0．5％羧甲基纤维素钠；对乙酰氨基酚组于造模前30min一次性给予对乙酰氨基酚（50mg·kg^-1，ip）。肝组织髓过氧化物酶（MPO）活性采用MPO试剂盒检测。肝脏组织血红素氧化酶1（HO-1）和脑组织环氧合酶2（COX-2）蛋白表达采用Western印迹方法测定，血浆前列腺素E2（PGE2）含量用酶联免疫法检测，HO-1活性用Morita法检测。结果注射LPS后。模型组出现3相热，最高升温幅度为2．01℃。积雪草苷5，15和45mg·kg^-1组体温明显低于模型组，其中45mg·kg^-1组与对乙酰氨基酚组相当。与模型组比较，3个积雪草苷剂量组发热后6h肝组织MPO活性、脑组织COX-2蛋白表达和血浆中PGE，的产生明显降低，肝组织HO-1活性和蛋白表达升高。结论积雪草苷具有预防发热作用，该作用可能与抑制COX-2／PGE2系统，并抑制炎症因子MPO活性、增强炎症保护因子HO-1的活性和表达有关。

英文摘要：

AIM To explore the pyretolytic effect of asiaticoside. METHODS Fifty-six male SD rats were randomly divided into normal, model, vehicle, paracetamol and 3 doses of asiaticoside groups. Rat fever model was made by ip lipopolysaccharides （LPS, 100 μg· kg^-1）. The changes of the core temperature （AT） were tested every 30 min after ip LPS, and lasted for 6 h. The rats in asiaticoside （5, 15 and 45 mg· kg^-1 ） groups were ig asiaticoside for 3 d before ip LPS. The rats of normal and model groups were ig normal saline, and rats of vehicle group were ig 0.5% carboxymethyl cellulose, instead of asiaticoside. The rats of paracetamol group were ip paracetamol （50 mg· kg^-1） only once and 30 min later ip LPS. The myeloperoxidase （MPO） activity in liver was detected with the MPO kit. The protein expressions of cyclooxygenase-2 （ COX-2 ） in brain tissue and heme oxygenase-1 （ HO-1 ） in liver tissue were analyzed with Western-blot. The prostaglandin E2 （PGE2 ） content in plasma was detected with enzyme immunoassay and the activity of HO-1 in liver tissue was detected with Morita method. RESULTS The fever appearance of the model rats had three febrile phases, and the maximum amplitude of temperature achieved 2. 01 ℃. Compared with model group, the core temperatures of rats in asiaticoside （ 5,15 and 45 mg· kg^-1 ） groups were obviously lower, and asiaticoside 45 mg· kg^-1 group was similar to paracetamol group. Asiaticoside （5,15 and 45 mg· kg^-1） obviously reduced the protein expression of COX-2 in brain, MPO activity in liver and PGE2 content in plasma. The activity and expression of HO-1 in liver were elevated after ig asiaticoside （5.15 and 45 mg· kg^-1 ）. CONCLUSION Asiaticoside has the inhibitory effect on fever, and this action possibly is related to its inhibition of the COX-2/PGE2 system, reduction of MPO activity and enhancement of the activity and expression of HO-1.