中文摘要：

目的研究槲皮素对免疫球蛋白E（Ig E）介导的速发型过敏反应大鼠Ig E和白三烯（LT）水平、嗜酸性粒细胞（EOS）数量及肺组织炎症面积的影响。方法大鼠随机分为模型组,孟鲁司特钠组,槲皮素高（40 mg/kg）、低（10 mg/kg）剂量组。除空白组（未处理大鼠）给予生理盐水外,其他各组大鼠腹腔注射含氢氧化铝和卵白蛋白（OVA）的生理盐水,14 d后,尾静脉注射卵白蛋白（15 mg/kg）以诱发速发型过敏反应（3 d,1次/d）。大鼠血清中LT和Ig E水平采用ELISA法确定,肺泡灌洗液（BALF）和全血中EOS的数量通过细胞计数法统计,肺组织炎症面积采用病理学检查进行评价。结果槲皮素高、低剂量组大鼠血清中LT和Ig E含有量、BALF和全血中EOS的数量及肺组织炎症面积均明显低于模型组（P〈0.05,P〈0.01）。结论槲皮素可能通过抑制炎性介质释放、降低炎性反应而发挥抗速发型过敏反应的作用。

英文摘要：

AIM To investigate the effects of quercetin on the levels of Ig E and leukotriene（ LT）,the number of eosinophils（ EOS）,and the area of inflammation in pulmonary tissue in Ig E-induced type I allergy rats.METHODS The rats were randomly assigned to model,montelukast,high-dose（ 40 mg / kg） and low-dose（ 10 mg /kg） quercetin groups. Except for the control group（ untreated rats given with normal saline）,the rats in other groups were injected intraperitoneally with normal saline containing Al（ OH）3and OVA,and then administrated intravenously（ in tail vein） with 15 mg / kg OVA（ 3 d,once a day） after 14 d for inducing typeⅠallergy.The LT and Ig E levels in the serum of rats were assayed by ELISA,the numbers of EOS in BALF and whole blood were counted by cell counting,and the inflammation area in pulmonary tissue was evaluated by pathologic examination. RESULTS Compared with the model group,the levels of Ig E and LT in the serum,the numbers of EOS in BALF and whole blood,and the area of inflammation in pulmonary tissue were significantly reduced after being treated with high dose or low dose quercetin（ P〈0. 05,P〈0. 01）. CONCLUSION Quercetin＇s inhibition of type I allergy may be related to the suppression of inflammatory mediators＇ release and the reduction in inflammation reaction.