中文摘要：

【目的】明确茶尺蠖Ectropis obliqua Prout 5龄幼虫脑解剖结构,并分析和构建幼虫脑以及脑内部各神经髓结构的三维结构模型。【方法】采用免疫组织化学方法,利用突触蛋白抗体,染色标记脑内神经突触,定位突触联系密集分布的区域,获得脑内部神经髓的结构。利用激光共聚焦显微镜获取脑扫描图像,然后利用三维图像分析软件AMIRA进行图像分析,构建脑的三维结构模型,并计算脑以及脑内各神经髓结构的体积。【结果】突触蛋白抗体染色显示,茶尺蠖5龄幼虫脑内具有很多神经突触联系密集分布的区域,这些不同区域即为脑的不同神经髓结构。茶尺蠖幼虫脑主要包括前脑、中脑和后脑3个组成部分。其中前脑最大,包括成对的视叶、蕈形体、前脑桥和侧副叶以及不成对的中央体。视叶位于前脑的两侧后端。蕈形体位于脑半球正中间位置。侧副叶在中央体的下前方两侧。中央体在脑的正中心。前脑桥在中央体的上方后侧。除这些形态结构明显的神经髓区域外,前脑还包括大量内部边界不明显的神经髓区域,位于前脑左右两侧以及背侧和腹侧,这些区域被总称为中间脑,占整个脑神经髓的66%。触角叶为中脑的主要组成部分,在脑的下部最前端,为一对球状结构。后脑在脑的腹侧和触角叶下方,即围咽神经索进入脑的入口处。【结论】构建了茶尺蠖5龄幼虫脑以及各神经髓结构三维模型,分析了脑内各个神经髓之间的空间位置关系,明确了各神经髓的体积。茶尺蠖幼虫脑体积小而且结构简单的特征与其幼期视觉、嗅觉等感觉器官不发达、活动能力弱、行为简单的生物学习性相对应。

英文摘要：

【Aim】This study aims to investigate the neuropil structures of the brain of the 5th instar larva of the tea geometrid, Ectropis obliqua Prout and to reconstruct their three-dimensional models.【Methods】The immunohistochemistry with synapsin antibody was used to characterize the anatomy of the neuropil structures of the brain. The laser scanning confocal microscope was used to acquire the confocal image stacks of the brain and the digital three-dimensional reconstructions were created by using the image analysis software AMIRA. 【Results】The immunostaining with synapsin antibody revealed that there are many areas with dense synapse in the brain of the 5th instar larva of E. obliqua,which form different neuropil structures of brain. The larval brain of E. obliqua is composed of three main parts：protocerebrum,deutocerebrum and tritocerebrum. The protocerebrum contains several paired neuropils,including optic lobes,mushroom bodies,protocerebral bridges and lateral accessory lobes,and an unpaired neuropil of central body. The optic lobes are located posteriorly to the lateral parts of the protocerebrum. The mushroom body is located on the midline of each half brain. The central body is located in the center of brain. The protocerebral bridges are located dorso-posteriorly to the central body,while the lateral accessory lobes are located ventro-anteriorly to the central body. The protocerebrum is the largest part of the brain. In addition to these prominent neuropils,the protocerebrum at its lateral,ventral and dorsal parts also contains a midbrain which accounts for 66% of the brain neuropils. The boundaries of different parts of the midbrain, however, are too fuzzy to be discriminated. The deutocerebrum mainly consists of a pair of antennal lobes. The tritocerebrum at the entrance of circumoesophageal connectives into the brain is located in ventral part of the brain and under the antennal lobe. 【Conclusion】The three-dimensional models of the brain including the neuropils were reconstructed.The volume