10%杠柳根皮提取物微乳剂是一种新型植物源杀虫剂,测定其对非靶标生物的影响是农药登记和科学合理使用这一新型杀虫资源过程中必不可少的环节。依据“化学农药环境安全性评价试验准则”,测定该制剂对6种非靶标生物的毒性。结果表明:10%杠柳根皮提取物微乳剂对蜜蜂、家蚕、瓢虫均无触杀毒性,对蜜蜂、家蚕和瓢虫的胃毒LC50分别为2 600.42mg·L-1、3 148.09mg·L-1和1 540.29mg·L-1;对鹌鹑7d的急性经口LD50为2 736.07 mg·kg-1,蓄积毒性为轻度蓄积,蓄积系数为5.30;对鲤鱼72h的LC50为153.62mg·L-1;对蚯蚓14d的LC50为1 082.49mg·L-1。因此,10%杠柳根皮提取物微乳剂对6种供试非靶标生物均表现为低毒,是一种新型的环境友好型农药。
10% microemulsion of extracts from Periploca sepium (10% ME) is a kind of novel botani- cal insecticide, and its toxicity evaluation on non-target organisms is an essential link for registration and application. This study, based on the "Experimental Guideline for Environmental Safety Evalua- tion of Chemical Pesticide", investigated the toxicities of 10% ME on six non-target organisms. The results indicated that 10% ME showed non-contact toxicity to bee, silkworm and ladybug, and the LC50 for stomach poisoning activity were 2 600. 42 mg· L-1, 3 148. 09 mg· L-1 and 1 540. 29 mg·L-1 , respectively. The acute LC20 of 10% ME against quail was 2 736.07 mg· kg- 1 (7 d), and the cumulative toxicity was mild with cumulative coefficient of 5.30. The LC50 to carp and earthworm were 153.62 mg· L-1(72 h) and 1 082.49 nag· L-1(14 d), respectively. In conclusion, 10~ ME is safe to the six non-target organisms, which was considered as an environment-friendly pesticide.