中文摘要：

采用柱层析分离、高效液相色谱切分和生物活性追踪法，从透骨草PhrymaleptostachyaL．石油醚提取物中分离出2个具有杀虫活性的化合物（B1和B2），采用质谱、核磁共振氢谱和碳谱，并结合相关文献对其结构进行鉴定，发现其分别为双氧木脂素A和E。首次以粘虫Mythimnaseparata、槐尺蠖Semiothisacinerearia、小菜蛾Plutellaxylostella、小地老虎Agrotisypsilon和家蝇Muscadomestica为试虫，测定了这2个化舍物的杀虫活性。结果表明：化合物B．和B，对3龄粘虫24h的胃毒致死中浓度（LC50值）分别为34．9和52．8μg／mL，24h触杀致死中量（LD50值）分别为0．09和0．26μg／头；对3龄槐尺蠖24h的胃毒LC50值分别为66．1和1675μg/mL，触杀LD50值分别为0．049和I．33μg／头；对家蝇成虫24h的胃毒LC卯值分别为39．2和969μg／mL，触杀LD50值分别为0．047和1．12μg头。

英文摘要：

Two compounds,haedoxane A and E were isolated from the petroleum ether extract of the roots and stems of Phryma leptostachya L. by silica gel chromatograph, high pressure liquid chromatograph（HPLC） and bioassay-guided fractionation. The bioassay results showed that haedoxane A and E had stomach toxic activity with the LCs0 value of 24 h 34.9 and 52.8 μg/mL against 3rd instar larvae of Mythimna separata, against 3rd instar larvae of Semiothisa cinerearia with the LCs0 value of 24 h 66. 1 and 1 675 μg/mL, and against Musca domestica with the LC50 value of 24 h 39.2 and 969 μg/mL. Haedoxane A and E had topical toxic activity with the LD50 value of 0. 09 and