中文摘要：

目的观察复元醒脑汤对糖尿病脑梗死大鼠内皮祖细胞（EPCs）功能的干预作用。方法将120健康雄性sD大鼠按随机数字表法分为对照组、假手术组、模型组、治疗组，每组30只。采用腹腔注射链脲佐菌素（STZ）50mg／kg后给予高能量高脂肪饲料喂养2周的方法复制糖尿病大鼠模型；制模后由颈内动脉注入自体血栓复制糖尿脑梗死模型；假手术组注射生理盐水。制模后治疗组给予复元醒脑汤[人参10g（单煎）、三七10鼽石菖蒲12g、水蛭10g、益母草30g、生南星15g、制大黄9g（后下），浓度388g／L]10．4mL·kg^-1·d。灌胃，每日2次，连续7d；假手术组、模型组给予等量生理盐水灌胃，连续7d。取大鼠外周血10mL离心，吸取界面层的单核细胞，传代培养后用于实验。用流式细胞仪检测各组细胞CD31、CD34、血管内皮生长因子受体-1（FLK-1）和血管性血友病因子（vWF）的表达量，将具有双标阳性的细胞作为EPCs的阳性表达，用四甲基偶氮唑盐（MTY）比色法测定细胞增殖能力，并测定细胞迁移能力。结果假手术组EPCs表达量较对照组显著降低【FLK-1为（34．64±1．92）％比（41．68±1．53）％，vWF为（40．92±1．16）％比（5i．98±1．06）％]，EPCs增殖率培养1d起即较对照组显著降低[（0．014±0＋001）％比（0．021±0．001）％]，持续到培养30d[（0．084±0．009）％比（0．121±0．003）％]，EPCs迁移率亦较对照组显著降低[吸光度（A）值：32．80±4．15比62．20±3．27，均P〈0．05]；模型组EPCs表达量较假手术组显著降低（FLK-1为（30．19±2．00）％比（34．64±1．92）％，vWF为（38．54±2．19）％比（40．92±1．16）％]，细胞增殖率培养1d起即较假手术组显著升高[（0．009±0.001）％比（0．014±0．001）％]，持续到培养30d[（0．057±0．005）％比（0．084±0．009）％]，EPCs细胞迁移率均显著低于假手术组[?

英文摘要：

Objective To observe the interference effect of Fuyuan Xingnao decoction on the function of endothelial progenitor cells （EPCs） in rats with diabetic cerebral infarction. Methods 120 healthy male Sprague- Dawley （SD） rats were divided into control group, sham-operation group, model group and treatment group by random digits table, each n = 30. The rat diabetes mellitus model was reproduced by intraperitoneal injection of urea with streptozotocin （STZ） 50 mg/kg and afterward the animal was fed by high energy high-fat diet for 2 weeks; after the diabetic modeling, the diabetic cerebral infarction model was reproduced by the injection of autologous thrombus into the internal carotid artery; in the sham-operation group, an ophthalmic surgical tweezers was used to ligate the left carotid vein and artery, afterward a clip was applied to close the artery in the neck and normal saline was injected into the internal carotid artery. After diabetic cerebral infarction modeling, in treatment group, 10.4 mL · kg ^-1· d^-1 of Fuyuan Xingnao decoction [ingredients： ginseng 10 g （decocted separately）, notoginseng 10 g, acori graminei 12 g, leech 10 g, motherwort 30 g, south star 15 g, prepared rhubarb 9 g （added later in the boiling decoction） and the concentration was 388 g/L] was given by gastric lavage, twice a day for consecutive 7 days. In the sham-operation group and model group, a same amount of normal saline was given by gastric lavage for consecutive 7 days. The rat peripheral blood 10 mL was collected and centrifuged, the mononuclear cells at the interface layer were absorbed for subculture to harvest the EPCs ready to be used in this experiment. The expressions of CD31, CD34, vascular endothelial growth factor receptor （FLK-1） and yon willebrand factor （vWF） were detected bv the flow cvtometrv and the oositive cells with double labelinz wereconsidered to be EPCs positive expression. The cell proliferation ability was determined by methylthiazolyl tetrazolium （MTT） and the cell migra