中文摘要：

目的：探讨人参与骨髓间充质干细胞（BMSCs）移植联合治疗对脊髓损伤（SCI）大鼠功能恢复的影响。方法将48只成年雌性Wistar大鼠按随机数字表法分为4组，每组12只。假手术组：仅打开椎板暴露脊髓，不造成脊髓损伤；SCI模型组：采用改良的Allen撞击法复制SCI模型，术后不予以治疗；甲泼尼龙组：SCI术后静脉推注（静推）大剂量甲泼尼龙（30 mg/kg）冲击治疗，4 h后重复1次，此后静推甲泼尼龙30 mg/kg，每日2次，共3 d；人参＋BMSCs组：SCI术后服用人参超微粉碎颗粒300 mg/kg，每日2次，共20 d，伤后7 d于损伤处移植大鼠BMSCs 5μL，浓度为1×10^7个/μL。各实验组定时观察行为学改变（BBB评分），术后30 d在镀银染色下观察脊髓组织学的变化，并检测大鼠神经电生理感觉诱发电位（SEP）、运动诱发电位（MEP）水平。结果假手术组术后双后肢均出现暂时性活动迟缓，7 d后功能恢复接近正常。SCI模型组伤后出现双后肢瘫痪，而甲泼尼龙组与人参＋BMSCs组均有不同程度的功能恢复，以人参＋BMSCs组恢复较为明显，达到假手术组水平。与假手术组比较，SCI模型组术后不同时间点BBB评分均明显降低；与SCI模型组比较，甲泼尼龙组和人参＋BMSCs组BBB评分均明显升高，且以人参＋BMSCs组升高更显著（均P＜0.05），两组术后12 d开始差异即有统计学意义（分：5.23±1.22比3.61±1.03，P＜0.05）。脊髓组织学检测显示：假手术组脊髓组织结构完整，神经元在灰质中分布均匀，可见大量密集分布的银染阳性神经纤维，相互平行，规则排列；SCI模型组可见组织结构不完整，损伤区灰、白质缺损明显；甲泼尼龙组和人参＋BMSCs组组织坏死程度较SCI模型组有所减轻。神经电生理检测结果显示，SCI模型组已失去正常波形；甲泼尼龙组和人参＋BMSCs组治疗后SEP与MEP潜伏期均较SCI模型组有不?

英文摘要：

Objective To explore the effect of ginseng combined with transplantation of bone marrow mesenchymal stem cells（BMSCs）on functional recovery of rats with spinal cord injury（SCI）. Methods Forty-eight female Wistar rats were randomly assigned to following four groups（n=12 per group）：the sham operation group was treated by opening the vertebral lamina and exposing spinal cord without SCI;the SCI model group was reproduced by using improved Allen bump method and afterwards no treatment was given;methyl prednisolone（MP）group was treated by MP pulse treatment after SCI,including intravenous injection of MP 30 mg/kg immediately after SCI and 4 hours later the same injection was repeated,and then the same intravenous injection 2 times daily,3 days in total;The ginseng＋BMSCs group was treated by orally taking ginseng ultra-microgranules 300 mg/kg after SCI,twice a day for 20 days and BMSCs 5μL（concentration 1×10^7 cell/μL）transplantation was carried out in SCI region on the 7th day after SCI. In the above 4 groups,the ethological observation（BBB scores）was done regularly and on the 30th day after operation,silver staining was applied to investigate the changes of spinal cord,and neuro-electrophysiological tests including somatosensory evoked potential（SEP）and motor evoked potential（MEP）were performed. Results In sham operation group,after surgery the movement of both hind limbs became temporarily sluggish and on the 7th day their functions recovered to approximately normal. In SCI model group,after injury paralysis of both hind limbs occurred,while in the MP group and ginseng＋BMSCs group,different degrees of functional recovery of the injured limbs developed,and the recovery in ginseng ＋ BMSCs group was more significant. Compared with sham operation group,after surgery the BBB score was reduced markedly at various time points in SCI model group;compared to the SCI model group,the BBB scores in MP and ginseng＋BMSCs groups were increased significantly,especially more remarkable in