中文摘要：

目的：研究蟾酥对豚鼠离体心脏的毒性作用和其物质基础。方法：通过Langendorff灌流研究蟾酥对豚鼠离体心脏的毒性作用,HPLC测定灌流后心脏中蟾蜍甾烯的含量。结果：蟾酥诱导豚鼠离体心脏出现房室传导阻滞、室速、室颤多种心律失常现象。当给药量累积达（60±11.5）μg时致其停跳。采用HPLC在灌流心脏中测到9种蟾蜍甾烯类化合物,其与心脏亲和力增加的顺序为：沙蟾毒精〉蟾毒灵〉远华蟾毒配基〉嚏根草苷元〉日蟾毒它灵〉华蟾毒灵〉蟾毒它灵〉脂蟾毒配基〉去乙酰蟾毒它灵。结论：蟾酥对豚鼠心脏具有显著毒性,其物质基础可能是脂蟾毒配基、蟾毒灵、蟾毒它灵、日蟾毒灵等9种蟾蜍甾烯类化合物。

英文摘要：

Objective：To study toad venom（BV）-induced cardiac toxicity and the toxic substances in isolated guinea-pig heart.Method：Isolated guinea pig hearts were switched to the Langendorff mode and perfused with or without BV.Measure the content of toad venom in perfused hearts using HPLC.Result：BV caused auriculo-ventricular block,supraventicular tachycardia,ventricular tachycardia,ventricular fibrillation and other arrhythmias phenomenon in isolated hearts.The cumulative doses of bufadienolides（60±11.5）μg induced cardiac arrest.Nine kinds of bufadienolides were detected by HPLC in the isolated hearts.Compare their affinity with the hearts：arenobufaginbufalintelocinobufaginhellebrigeningamabufotalincinobufalinbufotalinresibufogenindesacety-bufotalin.Conclusion：BV has cardiotoxicity on the guinea pig heart and the material basis of toad induced toxic effects may be gamabufotalin,arenobufagin and so on.