丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)由于不能纯培养,其分子生物学研究起步较晚。Simon等(1992)首次分析了AMFl8S核糖体基因,得到了第一条DNA序列,Wyss(1993)用RAPD-PCR技术得到AMF的RAPDs,Sanders等(1995)对AMF群落的孢子进行遗传分析,发现其孢子间存在着rDNA序列的多型现象,uoyd-MacGilp等1996研究证实了Glomus mosseae种间及其近缘种之间rRNA的ITS基因序列存在多样性。
The intra- or inter-species genetic diversity of 11 strain AMF from five host trees were studied by random amplified polymorphic DNA(RAPD). The genetic diversity among AMF species from different host plants were more abundant than from same host plants. The results showed that the genetic diversity is related to AMF species and host plants, whereas the efficacy of AMF species is higher than that of host plants; the same species AMF having same origination, there were different morphology and genetic divisions because of the choice of hosts and the menace of environment, which leaded to form different affinity between AMF and trees.