中文摘要：

目的：研究维生素E亚微粒乳液（VitESME）对中波紫外线损伤永生化角质形成细胞HaCaT株的光保护作用。方法：高压均质法加微乳法制备VitESME，稀释后加入培养基中孵育HaCaT株，高效液相色谱法测定不同时段培养液中VitE含量；中波紫外线（UVB）照射HaCaT株，孵育24h后四甲基偶氮唑蓝（MTT）法检测细胞活性。结果：培养液中VitE含量在孵育8h后即明显下降，24h后已减少90％以上。VitESME孵育24h后细胞增殖较为明显，与对照组相比活性增加了44．22％。与非加药照光组相比，加药照光组孵育24h后细胞增殖活性在30mJ／cm^2，下降17．77％，在90mJ／cm^2下降40．42％。结论：VitE SME对细胞培养体系无毒性作用，且具有优良的释放性能和通透性。VitE SME对UVB照射的保护作用呈时间递增性；预先加入VitE SME可以部分减少紫外线介导的损伤。

英文摘要：

Objective： The purpose of this study is to clarify the roles of tocopherol submicron emulsion （VitE SME） in UVB-induced damage to HaCaT keratinocytes, Methods： VitE SME was prepared by high pressure homogenization and microemulsion technique, HaCaT keratinocytes were incubated in the culture medium supplemented with 1/200 and 1/400 of VitE SME before different dosages of UVB irradiation. The VitE level in the culture medium was measured by HPLC. Cell growth and cellular activity were detected by MTY assay. Results： The VitE level in the culture medium significantly decreased during the first 8.h, and less than 10% can be detected by 24 h. In contrast to control group, the cellular activity of VitE SME treated group increased 44.22% by 24 h. Compared with irradiated groups without VitE SME, irradiated groups with VitE SME the cell proliferation decreased by 17.77% and 40.42% when irradiated with 30mJ/cm^2 and 90mJ/cm^2, respectively. Conclusions： VitE SME has no toxicity to cell culture system and has good releasing and penetrating ability. The photoprotective efficiency of VitE SME to UVB also shows time-dependent increase. Pre-incubation with VitE SME can partly reduce UV-induced cell damage.