中文摘要：

HCMV是一种广泛存在的疱疹病毒，在免疫抑制和免疫功能低下人群中，HCMV感染可引起严重疾病。RNaseP是细胞内催化tRNA5’末端成熟的酶，当EGSs与靶mRNA互补结合并形成类似tNRA的复合物时，大肠杆菌RNaseP催化亚基M1RNA可具备对靶mRNA特异的催化切割活性。为研究抗病毒制剂，针对HCMVDNA多聚酶UL54mRNA设计并构建特异性的EGS—C6，通过对觇舅基因亚克隆片段转录产物体外切割研究，证实该EGS具备引导M1RNA对UL54mRNA特异切割的能力，可发展成一种新型抗病毒制剂。

英文摘要：

HCMV is a ubiquitous herpesvirus. Infections by this virus can cause severe health problems in immunosuppressed and immunoeompromised individuals. RNase P is an enzyme which catalyzes a hydrolysis reaction to remove the leader sequence of precursor tRNA. When external guide sequences hybridize to the mRNA to form a structure resembling a tRNA substrate, M1 RNA from Escherichia coli can cleave the target mRNA specifically. To research antiviral agents, a sequence-specific EGS-C6 was used to target UL54 mRNA encoding major DNA polymerase of human cytomegalovirus. The results showed that EGS-C6 could direct the specific cleavage of UL54 mRNA in vitro and could be developed as a novel antiviral agent.