中文摘要：

工厂高度和 tillering 是决定米饭工厂建筑学和影响米饭谷物生产的关键因素。在这研究， multi-tillering dwarf1 (mtd1 ) ，稳定的到多为止和矮子异种，从 ethylmethane 被屏蔽对待 sulfonate 的装饰用的梨树米饭变化 Wuyunging7。与野类型相比， mtd1 异种展出了多种的显型，包括侏儒症，更多的 tillers，易碎的灰煤杆和推迟的标题日期。由采用基于地图的克隆的策略，基因 MTD1 最后在染色体 9 的短手臂上被印射到一个近似 66-kb 区域。定序结果证明在 mtd1 异种的基因 LOC_Os09g02650 (BC12 ) 有单个核苷酸替换(到 A 的 G ) ，它产生了一个早熟的翻译站。编码顺序的过去表示的 MTD1/BC12 包括植物高度和 tillers 救了 mtd1 异种的所有显型，它证实 BC12 是在 mtd1 异种的变异的基因。量的反向的 transcription-PCR 分析证明 MTD1/BC12 能否定地调整 MONOCULM 1，理想的植物 ARCHITECTURE1 和 Tillering 的表达式并且使 1 相形见绌，并且控制米饭 tillering。显著地，淀粉酶活动分析和 gibberellic 酸(GA ) 处理证明 mtd1 异种的矮子显型依赖于 GA 生合成小径。这些结果便于进一步揭开在米饭的生长和发展的分子的机制。

英文摘要：

Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtdl mutant exhibited pleiotropic phenotypes, including dwarf- ism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOCOsO9g02650 （BC12） in mtdl mutant had a single nucleotide substitution （G to A）, which gen- erated a premature translation stop. Over-expressing MTD1/BC12 coding sequ（nce rescued all the phenotypes of mtdl mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtdl mutant. Quantitative reverse tran,-eription-PCR analysis showed that MTDI/BCI2 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remark- ably, a-amylase activity analysis and gibberellic acid （GA） treatment showed that the dwarf phenotype of mtdl mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.