中文摘要：

采用东方香蒲（Typha orientalis Presl）和铜绿微囊藻（Microcystis aeruginosa）共培养的试验方法研究了东方香蒲对铜绿微囊藻多糖及亚显微结构的影响。结果表明：在质量浓度为20 g/L和40 g/L的东方香蒲化感胁迫下,铜绿微囊藻总糖（TSP）、胞内多糖（IPS）及固着性胞外多糖（bEPS）含量呈现先增加后逐渐降低的趋势,溶解性胞外多糖（sEPS）含量在培养过程中呈现逐渐增加的趋势,表明东方香蒲化感胁迫在前期促进了铜绿微囊藻的多糖合成和分泌,但在培养后期抑制多糖合成,并进一步促进多糖分泌和溶解;在培养后期,藻细胞叶绿素荧光参数包括YⅡ（光系统Ⅱ有效量子产量）、ETRmax（线性最大电子传递速率）显著降低,表明东方香蒲对铜绿微囊藻的光合系统产生了明显的化感抑制,光合活性及光合效率明显下降;藻细胞亚显微结构显示,在培养后期,藻细胞类囊体片层分解断裂,表明经过持续地化感胁迫,东方香蒲诱导铜绿微囊藻产生氧化胁迫,藻的光合系统受到严重破坏。东方香蒲使用量越高,藻细胞结构损伤越严重。

英文摘要：

In this paper,the author intends to investigate the effects of Typha orientalis Presl on the polysaccharide contents and the ultrastructure of the Microcystis aeruginosa by using the Typha orientalis Presl co-cultivated method with the Microcystis aeruginosa.What we want to determine is the content concentration rates of the polysaccharide total polysaccharide（TPS）,the intracellular polysaccharide（IPS） and the extra-cellular polysaccharide（EPS） in addition to the chlorophyll fluorescence parameters including Y Ⅱ（effective quantum yield of PS Ⅱ） and ETRmax（linear maximum electronic transferring rate） in the said ultrastructure.The results of our investigation and observation have shown that under the allelopathic stress of 20 g/L or 40 g/L of T.orientalis Presl,the synthesis and secretion of polysaccharide tends to be accelerated at first and then the process would become inhibited.In such a situation it would be necessary for us to stimulate its secretion and dissolution.What is more,in the process of incubation,the contents of the total compound of the polysaccharide,including the intracellular polysaccharide as well as the bound extracellular polysaccharide（bEPS） of M.aeruginosa can be found increasing at first and then began to decrease gradually afterwards.But the soluble extracellular polysaccharide（sEPS）has been found to show an increasing trend with the culturing process going on.However,the chlorophyll fluorescence parameters,such as Y Ⅱ and ETR_（max） of M.aeruginosa,tend to decrease significantly in the foregoing-on cultivation,which may imply that T.orientalis Presl has a significant allelopathical inhibition function for the photosynthetical system of M.aeruginosa,and in turn may result in a kind of photosynthetical activity and efficiency of M.aeruginosa.Besides,the ultrastructure of algal cells indicates that,the thylakoid of M.aeruginosa would begin to decompose 16 days later since the beginning of incubation with 20 g/L T.orientalis Presl,whereas the thylakoid