中文摘要：

以构建重组菌（JM109/pBV-220-GLZ）作为耐热α-葡萄糖苷酶的生产菌株,研究了诱导温度、诱导时间以及重组菌生长的不同阶段对重组菌生产耐热α-葡萄糖苷酶的影响,并对诱导表达的重组酶进行了固定化研究。结果表明,最佳的诱导条件是：诱导温度为40℃,诱导时间为14 h,在重组菌D（600）为2.0时诱导的酶活最高。适宜的固定化条件为：海藻酸钠20 g/L,CaCl220 g/L,硬化时间2 h,前交联剂戊二醛的质量分数为1.0%,该条件下的酶回收率高达81%。制备的固定化酶有较好的储存稳定性和操作稳定性,重复操作15次后,酶活为初始酶活的75%。

英文摘要：

Several factors influencing α-glucosidase production with the recombinant Escherichia coli haroring the JM109/pBV-220-GLZ were investigated. The results showed that the best inducement conditions were as follows： induc- tion temperature 40 ℃, induction time 14 h, and at OD600 of 2.0. To determine the optimum conditions of immobiliza- tion, the concentration of sodium alginate, CaCl2 , and glutaraldehyde, and solidifying time were studied. The results showed that the optimum operations were ： 20 g/L sodium alginate, 20 g/L CaCl2, 1.0 % glutaraldehyde, solidifying time 2 h. Under the optimum condition, the recovery rate reached 81%. After 15 times repeated operations, the en- zyme activity remained 75 %, showing good operational stability. Thus, the superior stability of the recombinant enzyme laid the base for large-scale application.