中文摘要：

目的：研究人疱疹病毒6型（human herpesvirus-6,HHV-6）感染与神经胶质瘤的关系。方法：巢式PCR法检测40例神经胶质瘤样本和13例正常脑组织样本中HHV-6、人巨细胞病毒（human cytomegalovirus,HCMV）、HHV-7 DNA序列。免疫组化染色法检测神经胶质瘤样本和正常脑组织样本中HHV-6、HCMV、HHV-7抗原的表达。结果：巢式PCR法检测结果显示,神经胶质瘤组织HHV-6 DNA阳性率为42.5%,正常脑组织HHV-6 DNA阳性率为7.7%（P=0.020）,神经胶质瘤组织HCMV、HHV-7DNA阳性率分别为20.0%和5.0%,正常脑组织未检测到HCMV和HHV-7 DNA（P值分别为0.087和0.566）。进一步用免疫组化染色法检测HHV-6早期抗原p41的表达,神经胶质瘤组织阳性率为27.5%,正常脑组织未见阳性表达（P=0.030）。同时检测HHV-6晚期抗原gp116/64/54的表达,神经胶质瘤组织阳性率为32.5%,正常脑组织未见阳性表达（P=0.014）。用HCMV pp65抗体进行免疫组化染色后发现其在神经胶质瘤中阳性率为12.5%,正常脑组织未见阳性表达（P=0.229）。HHV-7 pp85抗原在神经胶质瘤样本和正常脑组织样本中均未检出阳性表达。结论：根据PCR和免疫组化的检测结果,HHV-6感染在神经胶质瘤和正常脑组织中具有显著性差异,HHV-6感染在神经胶质瘤的病因和发展过程中起了一定作用。

英文摘要：

Objective： To study the relationship between human herpesvirus（HHV）-6 infection and glioma.Methods：HHV-6,human cytomegalovirus（HCMV） and HHV-7 DNA in glioma samples（n = 40） and control samples（n = 13） were detected by nested PCR.The expression of HHV-6,HCMV and HHV-7 was detected by immunohistochemistry（IHC）.Results：HHV-6 DNA was positive in 17/40（42.5%） of glioma samples and 1/13（7.7%） of normal brain samples（P = 0.020）,while the positive rates of HCMV and HHV-7 DNA were 20.0% and 5.0% in glioma,respectively.HCMV and HHV-7 DNA hadn’t been found in normal brain samples（P = 0.087 and 0.566 respectively）.HHV-6 late antigen p41 was expressed in 11/40（27.5%） of tumors and 0 of the controls（P = 0.030）,while HHV-6 early antigen gp116/64/54 was expressed in 13/40（32.5%） of tumors and 0 of the controls（P = 0.014）.HCMV pp65 antigen was expressed in 5/40（12.5%） of glioma samples and 0/13 of the normal brain controls（P = 0.229）.HHV-7 pp85 antigen hadn’t been found in tumors and normal brain samples.Conclusion：Based on the results of nested PCR and immunohistochemistry,significant difference of HHV-6 infection between glioma and normal brain tissue was confirmed.HHV-6 infection may play a role on the pathagenesis and progression of glioma.