中文摘要：

目的调查人群中人疱疹病毒（HHV）,包括人巨细胞病毒（HCMV）、EB病毒（EBV）、HHV-6和HHV-7的感染情况。方法收集55例南京市无偿献血者的外周血标本,提取全血DNA,巢氏PCR扩增HCMV、EBV、HHV-6和HHV-7特异性序列,1%琼脂糖凝胶电泳鉴定。结果 55例献血者中EBV、HCMV、HHV-6和HHV-7的阳性感染率分别为50.9%、5.5%、49.1%和21.8%,并且存在多种病毒混合感染。结论 HHV筛查对临床相关疾病的诊治具有十分重要的意义,应引起医务工作者的重视。

英文摘要：

Objective To investigate the prevalence of four kinds of human herpes viruses （HHV）in the blood of blood donors, including human cytomegalovirus （HCMV）, epstein-barr virus （EBV）, HHV-6, HHV7. Methods DNA was prepared in whole blood samples collected from 55 blood donors. The presence of DNA of EBV, HCMV, HHV-6, HHV 7 were examined by specific nested-polymerase chain reaction. Results Prevalence of EBV, HCMV, HHV-6 and HHV-7 in whole blood were 50.9 %, 5.5 %, 49.1% and 21.8% respectively. Multi-infections were observed in some objects. Conclusion The examination of HHV infection is useful in diagnosis and treatment of HHV related disease.