中文摘要：

目的了解完整迷走神经刺激（IVNS）对大鼠全身炎性反应的影响。方法以内毒素血症为全身炎性反应模型。将100只SD大鼠随机分为A组[静脉注射内毒素／脂多糖（LPS）]、B组（切断迷走神经、注射LPS后对其远端行电刺激）、C组（注射LPS后行迷走神经干电刺激）、D组（除注射等量等渗盐水外，同C组处理）、E组（仅注射等量等渗盐水）。于注射前及注射后2、4、6h，观察血清肿瘤坏死因子α（TNF—α）、白细胞介素10（IL-10）水平变化。每组取5只大鼠测定平均动脉压（MAP）。结果注射后10min，前3组大鼠MAP值显著升高（P〈0．05），以A组最明显[（134．4±7．3）mmHg，1mmHg=0．133kPa，P〈0．05]；注射后30min，前3组MAP值明显下降（P〈0．05）；注射后10min～6h，A组MAP值均明显低于B、C组（P〈0．05）。注射后2、4、6h，B、C组血清TNF—α水平均明显低于A组（P〈0．05）。注射后4、6h，C组大鼠IL-10水平显著高于A、B组（P〈0．05）。结论IVNS能稳定全身炎性反应时的血流动力学指征，并具有抗炎效应。

英文摘要：

Objective To observe the effects of stimulation of intact vagus nerve （IVNS） on systemic inflammatory response in rats. Methods The model of systemic inflammatory response was reproduced by lipopolysaeeharide（LPS）. One hunderd Sprague Dawley rats were randomly divided into A group （with intravenous injection of LPS） , B group （with stimulation of efferent nerve trunk of vagus nerve after intravenous injection of lipopolysaeeharide and vagotomy ） ,C group （with stimulation of intact vagus nerve after intravenous injection of LPS ）, D group （ with vagus nerve stimulation after injection of equivalent isotonic saline ） , E group （with intravenous injection of equivalent isotonic saline）,with 20 rats in each group. Five rats of each group were used to determine mean aortic pressure（MAP） before injection andl0 min, 0.5 h, 1 h, 2 h, 4 h and 6 h after injection. The serum levels of tumor necrosis factor （TNF）ctand interleukin （IL）10 were determined with enzyme linked immunosorbent assay before injection and 2, 4 and 6 hour after injection. Results The level of MAP rose in A,B,C groups at 10 min after injection,especially in A group （134.40±7.3 mm Hg,1 mm Hg=0.033 kPa, P 〈0.05）,but it dropped in above groups at 30 min after injection. The level of MAP in A group was obviously lower than that in B, C groups during 10 min -6 h after injection . The serum level of TNF-α in A group was significantly higher than that in B,C groups at 2,4,6 hours after injection（ P 〈0.05）. Compared with that in C group, the serum level of IL-10 in A,B groups lowered markedly at 4,6 hours after injection （ P 〈 0.05 ）. Conclusion IVNS can stabilize hemodynamies and exert have anti-inflammatory effects at early stage of systemic inflammatory response.