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Genome-wide search for segregation distortion loci associated with the expression of complex traits in Populus tomentosa
  • ISSN号:2095-6355
  • 期刊名称:《森林生态系统:英文版》
  • 时间:0
  • 分类:S792.117[农业科学—林木遗传育种;农业科学—林学] S718.46[农业科学—林学]
  • 作者机构:[1]Key Laboratory of Genetics and Breeding in Forest Trees and Omamental Plants, Ministry of Education, Institute of Populus tomentosa, Beijing Forestry University, Beijing 100083, P. R. China, [2]Beijing Agricultural College, Beijing 102206, E R. China
  • 相关基金:Acknowledgement This work was financially supported by the National Natural Science Foundation ,of China (Grant Nos. 30571516 and 30170780)
中文摘要:

分子的标记的分离失真在大量有机体被报导了。它在在克隆鈥淟M 之间穿过 50 鈥 ?(Populus tomentosa ) 和它的混合克隆鈥淭B 控制建立的一个种间的 BC1 Populus 家谱被检测了 01 鈥 ?(P。tomentosa 脳 P。bolleana ) 。有 150 个 AFLP 标记(约 18.9% 全部的 loci ) 的一个总数的学习从孟德尔的比率(1:1 ) 展出了重要偏差(p < 0.01 ) 。百分之 25 个标记在克隆鈥淟M 的父母特定的基因连接地图上被印射 50 鈥 ? 和鈥淭B 有印射策略的 pseudo-test-cross 的 01 鈥 ? 。12 个连接组与扭曲的分离比率有标记,但是主要区域在克隆鈥淟M 的连接地图在连接组 TLG2, TLG4 和 TLG6 上 50 鈥吗?我们也用 Mapmaker/QTL 软件分析了在复杂特点的弄歪的 loci 和表示之间的协会。影响 12 个特点的 16 通常认为的 QTL 的一个总数在七个连接组上在弄歪的区域被识别。因此,我们能沿着全部染色体检测扭曲的 loci 的分发并且经由在种间的 BC1 P 的基因印射识别在量的特点和分离 loci 之间的协会。tomentosa 家庭。而且,基因性质和为在女、男的父母之间的区别的这些分离失真的可能的原因也被讨论。

英文摘要:

Segregation distortion of molecular markers has been reported in a broad range of organisms. It has been detected in an interspecific BC1 Populus pedigree established by controlled crossing between clone "LM50" (Populus tomentosa) and its hybrid clone "TB01" (P. tomentosa × p. bolleana). The study with a total of 150 AFLP markers (approximately 18.9% of the total loci) exhibited significant deviation from the Mendelian ratio (1:1) (p〈0.01). Twenty-five percent of the markers were mapped on the parental specific genetic linkage maps of clones "LM50" and "TB01" with a pseudo-test-cross mapping strategy. Twelve linkage groups had markers with skewed segregation ratios, but the major regions were on linkage groups TLG2, TLG4 and TLG6 in the linkage map of clone "LM50". We also analyzed the association between distorted loci and expression of complex traits with Mapmaker/QTL software. A total of 16 putative QTLs affecting 12 traits were identified in the distorted regions on seven linkage groups. Therefore we could detect the distribution of skewed loci along the entire genome and identify the association between quantitative traits and segregation loci via genetic mapping in an interspecific BC1 P. tomentosa family. Furthermore, the genetic nature and possible causes of these segregation distortions for differentiation between female and male parents were also discussed.

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期刊信息
  • 《森林生态系统:英文版》
  • 主管单位:中华人民共和国教育部
  • 主办单位:北京林业大学
  • 主编:尹伟伦
  • 地址:北京市海淀区清华东路35号
  • 邮编:100083
  • 邮箱:editorial@forestecosyst.com
  • 电话:010-82373371
  • 国际标准刊号:ISSN:2095-6355
  • 国内统一刊号:ISSN:10-1166/S
  • 邮发代号:80-536
  • 获奖情况:
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  • 被引量:0