中文摘要：

从 Pinus koraiensis 和 Pinussylvestriformis 的根围土壤的微生物脱氧核糖核酸被朊酶 K 基于 SDS 方法， CTAB 方法， PVP ( polyvinylpolypyrrolidone )方法，和结冰并且融化的方法提取，从根围土壤的粗略的脱氧核糖核酸被分离方法净化，银祷告吸收方法，并且挤压 DNAgel 方法。方法的不同提取并且净化的结果被比较并且评估。结果显示为在根围的微生物脱氧核糖核酸的抽取的最好的方法与 1.0% 的高盐集中(w/v ) 基于 SDS 方法玷污 wasproteinse K NaCl，它能有效地消除胡敏酸和其它杂质。因为有效地移开棕色的事和胡敏酸，分离方法对从根围土壤的 purifyDNA 合适，净化的产品被适合到 PCR 扩大。挤压脱氧核糖核酸胶化方法也是有优点的一个好纯化方法在费用便宜、在使用有效。

英文摘要：

Microorganism DNA of rhizosphere soil from Pinus koraiensis and Pinus sylvestriformis were extracted by proteinase K based on SDS method, CTAB method, PVP （polyvinylpolypyrrolidone） method, and freezing and thawing method and the crude DNA from rhizosphere soil were purified by dialysis method, silver beads absorption method, and squeezing DNA gel method. The results of different extracting and purifying methods were compared and evaluated. Results indicated that the best method of extraction for microorganism DNA in rhizosphere soil was proteinse K based on SDS method with high salt concentration of 1.0% （w/v） NaCl, which could effectively eliminate humic acids and other impurities. The dialysis method was suitable to purify DNA from rhizosphere soil because of effectively removing brown matters and humic acids and the purified products were suited to PCR amplification. Squeezing DNA gel method was also a good purification method with the advantage of inexpensive in cost and efficient in use.