中文摘要：

目的：观察桃红四物汤对产后血瘀大鼠血清NO和子宫组织VEFG、PI3K/Akt通路的影响,探讨THSWD改善产后血瘀证的＂祛瘀生新＂作用及可能的作用机制。方法：采用SD早孕大鼠采用灌胃米索前列醇（100μg/kg）和米非司酮（8.3mg/kg）方法建立产后血瘀大鼠模型,随机分为模型组,阳性对照组,桃红四物汤大、中、小剂量组,LY294002组,桃红四物汤高剂量＋LY294002组,另取正常受孕大鼠作为正常对照组。正常对照组和模型组灌胃蒸馏水,其他组给相应药物,连续给药7d后,腹主动脉取血,分离子宫,用化学法测血清中的NO浓度;用免疫组化法测子宫中VEGF的表达;用Western blot法测子宫组织中Akt、p-Akt、e NOS的表达。结果：桃红四物汤大、中剂量组明显升高产后大鼠血清NO的含量（P〈0.01）,具有较明显的量效关系;显著促进子宫组织中VEGF的表达（P〈0.01）;Western blot结果显示,桃红四物汤能促进产后子宫组织中Akt磷酸化为p-Akt以及e NOS的表达。结论：桃红四物汤通过调节PI3K/Akt信号通路,促进e NOS的活化,促进NO、VEGF生成,产生＂祛瘀生新＂作用。

英文摘要：

Objective： To observe the effects of Taohong Siwu Decoction（THSWD） on the VEGF, NO expression and PI3K/Akt pathway alterations of medicine-induced incomplete-abortion in early pregnancy rats. Methods： Early pregnancy rats were intragastrically administrated with misoprostol（100μg/kg） and mifepristone（8.3mg/kg） to established the incompleteabortion model. The incomplete-abortion rats were randomly divided into blank control group, model group（the same volume of distilled water）, positive control group（at daily dose of 4.3g/kg Motherwort Particles）, THSWD-treated groups（at daily dose of 18.0, 9.0, 4.5g/kg）, LY294002 group and THSWD（dose of 18.0g/kg） ＋ LY294002 group. After the successive oral administration for 7 days, the levels of NO in serum and VEGF in uterine tissues were detected by NO Assay Kit and immunohistochemical assay, separately. Western blot to analyze expression of Akt, p-Akt, e NOS protein in uterine tissues. Results： THSWD significantly improved the content of NO in serum and the expression of VEGF, Akt, p-Akt, e NOS in uterine tissues were also improved. Conclusion： THSWD has the effect in markedly improving hemorheology, promoting NO and NOS synthesis. Its mechanism may be related to activating e NOS and regulating PI3K/Akt pathway.