中文摘要：

目的筛选新疆地区结核分枝杆菌临床分离株与国际标准株H37Rv之间的差异基因，初步分析这些基因的功能。方法利用抑制性消减杂交技术，通过两轮两相杂交，驱赶相同基因富集差异基因。结果正相抑制性消减杂交一共获得6条新疆地区临床分离株中存在的差异基因，虽然这些基因在H37Rv基因组中不存在，但与国际标准临床株CDC1551以及复合群国际标准株KMS中的对应基因高度同源，分别与编码多萜醇磷酸甘露糖基转移酶Ⅱ、单加氧酶黄素结合蛋白、酰基转移酶蛋白、染色体复制起始密码子、脂酰基载体蛋白以及5’-磷酸吡哆胺氧化酶的基因有关。结论新疆地区结核分枝杆菌临床分离株与国际标准株H37Rv之间存在差异基因，这些差异基因的功能可能与已知同源基因的功能相似：增强细菌胞壁蛋白的吸附能力、抵抗氮氧合酶消化、提高乙酰基辅酶A的合成能力、调控复制起始密码子、合成胞壁脂酰基载脂蛋白和促进5＇-磷酸吡哆胺氧化酶代谢。

英文摘要：

Objective To screen differential Mycobacterium tuberculosis genes between Xinjiang clinical strains and H37Rv by suppression subtractive hybridization （SSH）, and to analyze the function of these specifically pathopoiesis genes. Methods Both M. tuberculosis Xinjiang clinical strains and H37Rv as tester and driver each other, most identical genome was drived whereas some distinctive genes was remained and enriched by utilization SSH technique. Meanwhile through inserting differential genes to E. coli all of sequences that we have cloned were determined by BLAST in GenBank. The function of differential genes between M. tuberculosis H37Rv and Xinjiang clinical strains were analyzed. Results We cloned and analyzed six different DNA fragments that only existed in Xinjiang clinical strains. One is the fragment of a gene coding monooxygenase, flavin-binding family identified by Glimmer2. One fragment belongs to acyltransferase family protein. One for aminotransferase, class Ⅱ , acyl carrier protein. One fragment belongs to chromosomal replication initiator protein DNA and one for M. tuberculosis paralogous family 11-pyridoxamine 5＇-phosphate oxidase-related. Meanwhile, we cloned ten DNA fragments only in H37Rv. Conclusion SSH technique can efficiently screen differential genes in M. tuberculosis in Xinjiang clinical strains. They are possible key genes that M. tuberculosis survive and fortify virulence in real-environment as same as their homogenic genes, such as enhanced adsorbability in wall-held protein, counteracted digestion by nitro-oxygenase, elevated composition capability in the acyltransferase, control chromosomal replication initiator protein, synthesized aminotransferase acyl carrier protein and pyridoxamine 5 ＇-phosphate oxidase.