中文摘要：

目的观测陕西省岐山县食用碘盐干预前已成年健康居民的甲状腺功能指标生物参考区间变化。方法按照美国临床生化研究院（NACB）建议标准选取了从2012年1月至2014年12月期间在本院进行健康体检的1480名35~92岁健康中老年人作为研究对象,按照检测年度、性别分组,检测促甲状腺激素（thyrotropin hormone,TSH）、三碘甲状腺原氨酸（triiodothyronine,TT3）、甲状腺素（tetraiodothyronine,TT4）、游离三碘甲状腺原氨酸（free triiodothyronine,FT3）、游离甲状腺素（free tetraiodothyronine,FT4）、甲状腺球蛋白抗体（thyroglobulin antibody,TGAb）、甲状腺微粒体抗体（thyromicrosomal antibody,TMAb）七项指标,并建立和比较各分组间甲状腺功能指标生物参考区间的差异。结果本地区中老年男性人群甲状腺功能指标中TSH、TT3、TT4、TGAb、TMAb三个年度间均存在显著性差异,中老年女性人群甲状腺功能指标中只有TT3、TGAb、TMAb三个年度间存在显著性差异。其中,TGAb、TMAb两项指标随年度增长趋势明显,TGAb、TMAb的P95生物参考区间已由2012年的〈4%分别变为2014年的〈10%及〈8%,整体变化幅度达到或超过100%。结论 2012年至2014年期间,在社会经济环境、生活习惯、地理气候没有发生明显改变的情况下,岐山县研究人群甲状腺自身抗体基线水平的逐年增高,印证了人为干预因素（全民食盐加碘）对陕西省省内自身免疫性甲状腺相关疾病谱的长期影响。同时表明,实时监测并适时调整本地区甲状腺功能检测指标的生物参考区间,对于区域内相关疾病的准确诊断和流行趋势判断具有重要意义。

英文摘要：

Objective To explore the changes of reference range of thyroid hormones in aged population in Qishan area ,who had arrived at manhood before the natiolaal salt iodination intervention started. Methods From 2012 to 2014,1480 disease-free aged individuals（ 〉 35 age ）were selected and divided into three groups according to their participation year. The serum levels of TSH, TT3 , TT4, FT3 , FT4, TGAb and TMAb in all groups were detected by radioimmunoassay. A series of reference range of thyroid hormones were calculated by each group. Results In males, the TSH, TT3, TT4, TGAb and TMAb levels between these three years had significantly difference. In females, only TF3, TGAb and TMAb had significantly difference. In this study population, TGAb and TMAb refence limit P95 levels were significantly increased in study period,from both 4% to 10% for TGAb and 8% for TMAb. From 2012 to 2014, under these circumstances without changing in the socio-economic environment, lifestyle and climate in Qishan area, the baseline on thyroid autoantibody levels increased, confirmed risk factor of the long-term effect of autoimmune thyroid disease, which was correlated the human intervention of salt iodination in Shaanxi Province. Conclusion The results in this study indicate that monitoring and adjustment in time of the local biological reference range for thyroid hormones detection is important for the accurate diagnosis and prediction of epidemic trend of thyroid related diseases.