中文摘要：

肝的房间和 chito-san/gelatin 答案的混合物被扔免职技术使用通过 layer-by-layer 构造肝的类似物一自制设计。类似物的尺寸和房间集中能自由地被控制。约 90% 肝的房间在 0.2 Mpa 挤出压力下面仍然保持可行。在在动物前的 8 个星期测试的 vitro 有教养，在结构的肝的细胞维持了他们的显型并且不停地增殖，并且白朊和细胞的另外的分泌物增加了。绳索和象 hepaton 一样结构为 20 d 在文化以后被观察。这些结果显示肝的房间能直接被装配进 3D 可行结构并且膨胀了到形式一肝或硬鳞鱼。这成就被认为是为肝代替的制造的一个有趣的工具。

英文摘要：

A mixture of hepatic cells and chitosan/gelatin solution was deposited to construct a hepatic analog by way of layer-by-layer deposition technique using a home-made devise. The size and cell concentration of the analogs can be controlled freely. Approximately 90% of the hepatic cells remained viable under 0.2 Mpa extrusion pressure. Cultured in vitro 8 weeks before animal test, hepatic cells in structure maintained their phenotype and kept proliferating, and albumin and other secretion of the cells increased. Cords and hepaton-like structures were observed after culture for 20 d. These results indicate that hepatic cells could be assembled directly into a 3D viable structure and expanded to form a hepatic organoid. This accomplishment is considered to be an interesting means for the fabrication of liver replacements.