采用扫描电镜和光学显微镜观察了采自我国的蟾蜍属(Bufo)7种(亚种)的精子形态,对精子各部位量度进行了测量和计算。结果表明,该属7种(亚种)精子的形态基本相同,精子由头部、中片和尾部组成,头部细长微弯且前端渐尖,中片有球状突起,尾部长,由轴纤维、轴丝和波动膜构成。与已有报道的两栖动物的精子形态相比较,蟾蜍属精子与无尾类其他科精子形态差别较大,而与有尾类精子形态相似。本文认为两栖动物精子形态和量度在科间存在明显差异;两栖动物精子形态的差异可能与其繁殖模式有关。
Sperm shape of 7 species(subspecies)of the genus Bufo was observed by light and scanning electron microscope,and the size of spermatozoa were also measured.The results show that the sperm from the 7 species(subspecies)are basically similar in shape:the sperm are composed of head,mid-piece and tail;the head is thin,long and slightly curved;the mid-piece is relatively evident,and the tail is long and winding with an undulating membrane.The shape of spermatozoa from genus Bufo differs remarkably from that of other families of Anura,but is more similar to that of Urodela.The spermatozoa differ in morphology and size among different families of Amphibia,and the diversity of sperm morphology of Amphibia might be related to their different reproductive modes.