中文摘要：

【目的】探讨柔嫩艾美耳球虫（Eimeria tenella）入侵细胞与钙信号转导之间的关系，揭示鸡柔嫩艾美耳球虫病的发生机制。[方法]采用体外狗肾细胞（madin-darby canine kidneycell，MOCK细胞）培养技术，检测了细胞外缺钙、Ca2＋内流阻断剂（硝苯地平）和钙调蛋白抑制剂（三氟拉嗪）对E．tenella子孢子入侵率的影响，并测定了培养细胞上清液中乳酸脱氢酶（LDH）和细胞活性。【结果】E．tenella子孢子入侵细胞的抑制率随着细胞外Ca2＋浓度的降低而升高。钙离子浓度降低到600umol·L-1时，入侵率（23．33％）均极显著低于对照组（P〈0．01）；细胞外无钙时，入侵抑制率高达53．18％；硝苯地平和三氟拉嗪均能极显著抑制子孢子的入侵（P〈0．01），其中10umol·L-1的硝苯地平和50umol·L-1三氟拉嗪分别对子孢子的入侵抑制率达71．41％和97．13％，二者合用入侵抑制率可达98．59％。在E.tenella子孢子入侵细胞的过程中，MDCK细胞的活性均在90％以上，与对照组差异不显著（P〉0．05），接种最tenella子孢子的MOCK细胞培养液中LDH的活性与未接种的活性差异不显著（P〉0．05）。【结论】细胞外钙缺乏、钙通道阻断剂硝苯地平和钙调蛋白抑制剂三氟拉嗪对E．tenella子孢子入侵MOCK细胞均有抑制作用，但子孢子入侵对MDCK细胞的活性无明显影响。

英文摘要：

[Objective] The objective of the study is to investigate the relationship between Ca2＋ signal transduction and Eimeria tenella sporozoite invasion, and to reveal the mechanism of coccidiosis （E. tenella）. [ Method ] An assay system in vitro was used to study the effects of E. tenella sporozoites invasion into MDCK cell by extracellular Ca2＋ deficiency or Ca2＋ inflow blocker （nifedipine） or calmodulin inhibitor （trifluoperazine）. Viability of MDCK cells and lactate dehydrogenase in supernatant liquid was examined. [Result] The inhibition rate of E. tenella sporozoite invasion increased with the decrease of the Ca2＋ concentration. At low Ca2＋ concentration of 600 umol.L-1, the rate ofE. tenella sporozoite invasion （23.33%） was significantly （P〈 0.01） lower than that of the control group. When extracellular environment was absent of Ca2＋ absolutely, the inhibition rate rose to 53.18%. Either nifedipine or trifluoperazine could inhibit the E. tenella sporozoite invasion obviously （P〈0.01）, the inhibition rate of 71.41% and 97.13% were achieved by 10 umol·L-1 nifedipine and 50 umol·L-1 trifluoperazine, respectively. The inhibition rate of 98.59% was reached by nifedipine and trifluoperazine together. The viability of MDCK cells with E. tenella sporozoite invasion exceeded 90%, and the data was not remarkably different compared with the control group （P〉0.05）. The same is as the viability of lactate dehydrogenase in supernatant liquid. [Conclusion] Extracellular Ca2＋ deficiency, calciumion inflow blocker （nifedipine） and calmodulin inhibitor （trifluoperazine） could inhibit the E. tenella sporozoite invasion of MDCK cells. The host cell activity was not affected by E. tenella sporozoite invasion.