中文摘要：

目的 建立人破骨样细胞体外培养的方法 ,探讨1α,25-（OH）2D3、巨噬细胞集落刺激因子（M-CSF）、前列腺素E2（PGE2）等骨吸收刺激因子对破骨细胞分化、增殖和功能的影响,为进一步研究正畸牙齿移动的生物力学机制奠定基础。方法 将脐血单核细胞接种于预置盖玻片和骨片的24孔培养板中,实验组分别加入诱导因子1α,25-（OH）2D3、M-CSF、PGE2,对照组不加,每3 d换液一次,培养7 d。采用倒置相差显微镜、TRAP染色等方法 观察破骨样细胞的形成情况。结果 第3天实验组单核细胞出现融合趋势,第7天TRAP染色可见阳性的多核破骨样细胞,但尚未形成骨吸收陷窝,以1α,25-（OH）2D3组破骨样细胞形成数量最多。结论 脐血单核细胞经1α,25-（OH）2D3、M-CSF、PGE2体外诱导培养后可分化为TRAP（＋）多核的破骨样细胞,且10^-8mol/L的1α,25-（OH）2D3具有最强的生物学效应。

英文摘要：

Objective To establish a stable and useful method for culturing human osteoc1αst-like cells in vitro, and investigate the effect of 1α , 25-（OH）2D3, M-CSF and PGE2 on osteoc1αsts differentiation, proliferation and activation so as to 1αy the foundation for further study of the biological mechanism for tooth movement. Methods The HCMNC were iso1αted and cultured in 24-well p1αte with coverslips and human dentine slices. The experiment group was cultured with 1α , 25-（OH）2D3, M-CSF and PGE2, respectively, while the control group was not. The liquid was changed every 3 days and the whole culture process 1αsted for 7 days. The phase contrast microscopy and TRAP staining were adopted to identify osteoc1αst-like cells. Results On the 3^rd day the monocytes began to fuse and on the 7th day positive multinucleated cells could be seen with TRAP staining, but absorption pit was not formed on the dentin slices. The group with 1α , 25-（OH）2D3 had the 1αrgest number of osteoc1αst-like cells. Conclusion After the monocytes in UCB are cultured by 1α , 25-（OH）2D3, M-CSF, PGE2 induction, they can turn into TRAP（＋） multinucleate osteoc1αst-like cells, the 1α, 25-（OH）2D3 10^- 8mol/L being the most effective.