中文摘要：

目的探讨神经干细胞移植治疗颞叶癫痫的疗效。方法首先建立大鼠的海人酸颞叶癫痫模型。然后从胚胎大鼠的海马中分离培养出神经干细胞，将预诱导的、向氨基丁酸能神经元方向分化的胚胎神经干细胞移植入颞叶癫痫模型的海马内，在移植后1w、2w、3w和4w分别进行移植癫痫大鼠的癫痫最低阂值的测定、海马中氨基丁酸的测定和移植的神经干细胞在体内分化情况的鉴定。结果移植后的癫痫最低阈值和海马中的氨基丁酸逐渐增加，接近但未达到正常值，预诱导的神经干细胞全部分化为氨基丁酸能神经元。结论神经干细胞移植治疗颞叶癫痫具有一定效果。

英文摘要：

Objective To study therapeutic effect of transplantation of neural stem cells on temporal lobe epilepsy. Methods Kainic acid-induced rat models of temporal lobe epilepsy were established. Neural stem cells were obtained from rat embryo and induced to differentiate into neurons that produce γ-aminobutyric acid （GABAergic like neurons） in vitro. After GABAergic like neurons were transplanted into the hippoeampus of rat temporal lobe epilepsy models, the low threshold of epilepsy, the level of GABA and differentiation of neural stem cells in hippocampus were measured 1 w, 2 w, 3 w and 4 w after transplantation. Results The low threshold of epilepsy and the level of GABA were increasing gradually but didn＇t reach the normal level. All GABAergie like neurons induced in vitro differentiated into GABAerrgic neurons. Conclusion It is effective to treat temporal lobe epilepsy with the transplantation of neural stem cells.