中文摘要：

目的 探讨人表皮生长因子受体-2（HER-2/neu）特异性siRNA对高表达HER-2/neu的人胶质瘤细胞株T98G侵袭性的影响及其机制.方法 用脂质体介导终质量浓度50 nmol/LHER-2/neu siRNA转染人胶质瘤细胞株T98G,实时定量聚合酶链反应（real-time PCR）和免疫印迹法检测转染后HER-2/neu mRNA和蛋白表达变化.Transwell体外侵袭实验检测终质量浓度25、50、100 nmol/L HER-2/neu siRNA转染T98G后细胞侵袭能力的变化.免疫印迹法检测转染后细胞基质金属蛋白酶-9（MMP-9）蛋白表达变化.明胶酶谱法检测转染后MMP-9活性变化.结果 终质量浓度50 nmol/L HER-2/neu siRNA转染T98G细胞后HER-2/neu mRNA和蛋白表达为对照组的（29.3±6.2）%和（13.1±8.9）%（P＜0.01）.终质量浓度25、50、100 nmol/L HER-2/neu siRNA转染后细胞侵袭抑制率分别为（37.7 ±7.2）%、（65.0±10.1）%和（69.7±9.3）%,三者与对照组的差异均有统计学意义（P＜0.01）.与对照组比较,转染组细胞MMP-9蛋白表达减少（49.9±10.7）%,酶活性下降（55.6±12.7）%（P＜0.01）.结论 HER-2/neu siRNA转染人胶质瘤细胞株T98G后明显抑制细胞的侵袭能力,其机制与MMP-9蛋白表达和活性显著受到抑制有关.

英文摘要：

Objective To investigate the inhibitory effect of HER-2/neu siRNA on invasion of human glioma cell line T98G which over-express HER-2/neu, and to explore the possible mechanisms.Methods Specific siRNA with concentration of 50 nmol/L was transfected into human glioma cell line T98G to knockdown the expression of HER-2/neu by using lipofectamine. The changes of HER-2/neu mRNA and protein expressions were evaluated by real-time polymerase chain reaction （PCR） and Western blotting. After transfected by HER-2/neu siRNA with concentration of 25, 50, 100 nmol/L,the change of invasive capacity of the glioma cells was investigated by using Transwell assay. The effects of HER-2/neu siRNA on protein expression and enzyme activity of matrix metalloproteinase-9 （ MMP-9 ） were studied by using Western blotting and gelatin-containing zymography, respectively. Results HER-2/neu mRNA and protein expressions of transfected T98G cells were decreased to （29.3 ±6. 2）% and （ 13. 1 ± 8. 9）% as compared with those in control cells, respectively. After transfected by HER-2/neu siRNA with concentration of 25, 50, 100 nmol/L, the inhibition rates of invasion in transfected T98G cells were （37.7 ±7.2）%, （65.0± 10. 1）% and （69.7 ±9.3）%, respectively （as compared with control group, P〈0. 05）. Protein expression of MMP-9 was decreased by （49.9 ± 10.7）% and enzyme activity of MMP-9was reduced by （55.6 ± 12. 7）% in transfeeted cells （P 〈 0. 01 ）. Conclusion The specific siRNA silencing HER-2/neu in human glioma cell line T98G could inhibit cell invasive capacity involving reducing protein expression and enzyme activity of MMP-9.