中文摘要：

采用95％乙醇对甘遂进行提取，溶剂回收后得总浸膏；然后采用不同极性的有机溶剂进行萃取得萃取物；将总浸膏和各萃取物进行激活NF—KB信号通路活性检测；选取激活作用最强的氯仿萃取物进行硅胶柱层析，TLC检测合并；将得到的各部位进行进一步的激活NF—KB信号通路测试；结果表明激活作用最强的部位为非极性部位，因此我们采用GC—MS技术分离并鉴定了56个化合物，占总量的99．72％，主要成分为脂肪酸及其衍生物、长链烷烃及其衍生物，还有单萜、倍半萜类、芳香族类和脂肪醛等。在激活NF—KB信号通路的指导下，我们从甘遂非极性部位分析并鉴定了其中的促炎成分如（z）-7-Hexadecenal和7，11-Hexadecadienal等，这些成分激活NF．KB信号通路的表达，从而引起炎症因子的释放，导致炎症，从上述非极性部位中还分析了部分毒性物质，为阐明甘遂的毒效物质基础提供了参考。

英文摘要：

Kansui Radix, the dried roots of Euphorbia kansui Liou. （Euphorbiaceae）, are commonly used traditional Chi- nese medicine. It was reported that the usage of Kansuiwould cause inflammation. An NF-KB-Luc reporter system was es- tablished to evaluate the bioactivity of Kansuiin this study. The 95% ethanol crude extractand different solvent-soluble extracts of Kansui were obtained by solvent extraction and fractionation. Bioassay guided separation suggested that the first sub-fraction （ C1 ） of the CHC13-soluble fraction displayed strong bioactivity. GC-M$ was introduced to separate and identify the chemical components of the nonpolar sub-fraction of Kansui （ C1 ）. Totally 56 compounds were identified from the non-polar sub-fraction C1. The main components were aliphatie acids and derivatives, long-chain alkanes, monoterpenes, sesquiterlenes, aromatic eompoundsand aldehydydes. The aliphatic aldehydes such as （Z）-7-hexadecenal and 7,11-hexadecadienal detected in the sample extract were reported to cause inflammation. Some aromatic compounds were tumorigenic agents. The results in this paper provided a clue for the further investigation of toxic substances in Kansui.